Carbohydrate Microarrays in Plant Science

Jonatan Ulrik Fangel, H.L. Pedersen, S. Vidal-Melgosa, Louise Isager Ahl, A.A. Salmean, Jack Egelund, Maja Gro Rydahl, M.H. Clausen, William George Tycho Willats

Research output: Contribution to journalJournal articleResearchpeer-review

1 Downloads (Pure)

Abstract

Almost all plant cells are surrounded by glycan-rich cell walls, which form much of the plant body and collectively are the largest source of biomass on earth. Plants use polysaccharides for support, defense, signaling, cell adhesion, and as energy storage, and many plant glycans are also important industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities.
Original languageEnglish
JournalMethods in Molecular Biology
Volume918
Pages (from-to)351-362
ISSN1064-3745
DOIs
Publication statusPublished - 2012

Keywords

  • Carbohydrate microarrays
  • Glycans
  • High-throughput
  • Carbohydrate binding proteins
  • Carbohydrate binding modules
  • Polysaccharides
  • Oligosaccharides
  • Comprehensive microarray polymer
  • Defined glycan arrays

Fingerprint Dive into the research topics of 'Carbohydrate Microarrays in Plant Science'. Together they form a unique fingerprint.

Cite this