Capture of DNA in microfluidic channel using magnetic beads: increasing capture efficiency with integrated microfluidic mixer

Torsten Lund-Olesen, Hans Martin Dufva, Mikkel Fougt Hansen

    Research output: Contribution to journalConference articleResearchpeer-review

    Abstract

    We have studied the hybridization of target DNA in solution with probe DNA on magnetic beads immobilized on the channel sidewalls in a magnetic bead separator. The hybridization is carried out under a liquid flow and is diffusion limited. Two systems are compared: one with a straight microfluidic channel and one with an integrated staggered herringbone mixer. Fluorescence microscopy studies show that the hybridization is much more efficient in the system with the integrated mixer. The results, which are discussed in terms of a simple model, are relevant for any diffusion-limited reaction taking place on the surface in a microfluidic system.
    Original languageEnglish
    JournalJournal of Magnetism and Magnetic Materials
    Volume311
    Issue number1
    Pages (from-to)396-400
    ISSN0304-8853
    DOIs
    Publication statusPublished - Apr 2007
    EventInternational Conference on the Scientific and Clinical Applications of Magnetic Carriers - IMC University of Applied Science, Krems, Austria
    Duration: 17 May 200630 May 2006
    Conference number: 6

    Conference

    ConferenceInternational Conference on the Scientific and Clinical Applications of Magnetic Carriers
    Number6
    LocationIMC University of Applied Science
    CountryAustria
    CityKrems
    Period17/05/200630/05/2006

    Keywords

    • magnetic bead
    • magnetic separation
    • microfluidic
    • bioseparation
    • lab on a chip

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