Campylobacter in chicken – Critical parameters for international, multicentre evaluation of air sampling and detection methods

Gro S. Johannessen, Giuliano Garofolo, Gabriella Di Serafino, Ivana Koláčková, Renáta Karpíšková, Kinga Wieczorek, Jacek Osek, Julia Christensen, Mona Torp, Jeffrey Hoorfar*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The present pilot study aimed at evaluating air sampling as a novel method for monitoring Campylobacter in poultry farms. We compared the bacteriological isolation of Campylobacter from boot swabs and air filter samples using ISO 10272–1:2017. A secondary aim was to evaluate the use of molecular methods, i.e. real time PCR, on the same sample set. Samples from 44 flocks from five European countries were collected, and included air samples, in parallel with boot swabs.

Campylobacter spp. was isolated from seven of 44 boot swabs from three of five partners using the enrichment method. Two of these positive boot swab samples had corresponding positive air samples. Using enrichment, one positive air sample was negative in the corresponding boot swabs, but Campylobacter spp. was isolated from direct plating of the boot swab sample. One partner isolated Campylobacter spp. from six of 10 boot swabs using direct plating. Overall, 33 air filter samples were screened directly with PCR, returning 14 positive results. In conclusion, there was a lack of correspondence between results from analysis of boot swabs and air filters using ISO 10272–1:2017. In contrast, the combination of air filters and direct real-time PCR might be a way forward. Despite the use of the detailed ISO protocols, there were still sections that could be interpreted differently among laboratories. Air sampling may turn into a multi-purpose and low-cost sampling method that may be integrated into self-monitoring programs.
Original languageEnglish
Article number103455
JournalFood Microbiology
Volume90
Number of pages6
ISSN0740-0020
DOIs
Publication statusPublished - 2020

Keywords

  • Zoonoses
  • Food
  • Standardization
  • ISO
  • PCR
  • Air sampling

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