Campylobacter contamination level in houseflies after exposure to materials containing Campylobacter

Annette Nygaard Jensen*, Birthe Hald

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Although houseflies have been found to carry Campylobacter jejuni, little is known about the quantitative campylobacter level in naturally contaminated houseflies and their ability to contaminate surfaces. This study aimed to elucidate how houseflies’ previous exposure to campylobacter-contaminated material (faeces or liquid) for 1 or 4 h affects the acquired campylobacter level in houseflies and their contamination potential. Cups of 250 ml were added 5 g of chicken faeces or 1 ml liquid and spiked with approximately 3, 4, 5 or 7 log10 cfu C. jejuni. Sixteen houseflies were added to each cup. After 1 h of exposure, four houseflies were removed from the cup for enumeration of campylobacter in each fly by plate spreading. Another four houseflies were transferred onto Abeyta-Hunt-Bark (AHB) agar plates (9 cm) to assess possible contamination of surfaces. After 1 h on the AHB plate, each fly was tested for level of campylobacter. This procedure was repeated after approx. 4 h of exposure for the remaining eight houseflies. The C. jejuni acquisition in houseflies increased with exposure dose and was higher after liquid exposure compared with faeces exposure, while there was no significant effect of exposure time (1 vs 4 h). For faeces, 90.0% (n=80), 48.4% (n=64), 6.3% (n=48) and 0% (n=16) of houseflies were campylobacter-positive when exposed to 7, 5, 4, and 3 log10 cfu with a mean (±SE) of 2.0±0.1, 0.8±0.1, 0.3±0.0 and 0 log10 cfu recovered per campylobacter-positive fly, respectively. For liquid, 95.7% (n=47), 91.4% (n=47), 20.8% (n=48) and 6.3% (n=16) of houseflies were campylobacter-positive with a mean of 3.3±0.2, 2.0±0.1, 0.8±0.2 and 0.3±0.0 log10 cfu. The surface of the AHB plates was only contaminated by houseflies previously exposed to >4 log10, but the C. jejuni number found on the AHB surface did not correlate with the number found in the corresponding fly.
Original languageEnglish
JournalJournal of Insects as Food and Feed
Volume4
Issue number3
Pages (from-to)179-186
ISSN2352-4588
DOIs
Publication statusPublished - 2018

Keywords

  • Houseflies
  • Campylobacter transmission
  • Exposure level
  • Contamination

Cite this

@article{13a53b55a53f4d2193579c4562f5cd4a,
title = "Campylobacter contamination level in houseflies after exposure to materials containing Campylobacter",
abstract = "Although houseflies have been found to carry Campylobacter jejuni, little is known about the quantitative campylobacter level in naturally contaminated houseflies and their ability to contaminate surfaces. This study aimed to elucidate how houseflies’ previous exposure to campylobacter-contaminated material (faeces or liquid) for 1 or 4 h affects the acquired campylobacter level in houseflies and their contamination potential. Cups of 250 ml were added 5 g of chicken faeces or 1 ml liquid and spiked with approximately 3, 4, 5 or 7 log10 cfu C. jejuni. Sixteen houseflies were added to each cup. After 1 h of exposure, four houseflies were removed from the cup for enumeration of campylobacter in each fly by plate spreading. Another four houseflies were transferred onto Abeyta-Hunt-Bark (AHB) agar plates (9 cm) to assess possible contamination of surfaces. After 1 h on the AHB plate, each fly was tested for level of campylobacter. This procedure was repeated after approx. 4 h of exposure for the remaining eight houseflies. The C. jejuni acquisition in houseflies increased with exposure dose and was higher after liquid exposure compared with faeces exposure, while there was no significant effect of exposure time (1 vs 4 h). For faeces, 90.0{\%} (n=80), 48.4{\%} (n=64), 6.3{\%} (n=48) and 0{\%} (n=16) of houseflies were campylobacter-positive when exposed to 7, 5, 4, and 3 log10 cfu with a mean (±SE) of 2.0±0.1, 0.8±0.1, 0.3±0.0 and 0 log10 cfu recovered per campylobacter-positive fly, respectively. For liquid, 95.7{\%} (n=47), 91.4{\%} (n=47), 20.8{\%} (n=48) and 6.3{\%} (n=16) of houseflies were campylobacter-positive with a mean of 3.3±0.2, 2.0±0.1, 0.8±0.2 and 0.3±0.0 log10 cfu. The surface of the AHB plates was only contaminated by houseflies previously exposed to >4 log10, but the C. jejuni number found on the AHB surface did not correlate with the number found in the corresponding fly.",
keywords = "Houseflies, Campylobacter transmission, Exposure level, Contamination",
author = "{Nygaard Jensen}, Annette and Birthe Hald",
year = "2018",
doi = "10.3920/JIFF2018.0007",
language = "English",
volume = "4",
pages = "179--186",
journal = "Journal of Insects as Food and Feed",
issn = "2352-4588",
publisher = "Wageningen Academic Publishers",
number = "3",

}

Campylobacter contamination level in houseflies after exposure to materials containing Campylobacter. / Nygaard Jensen, Annette; Hald, Birthe.

In: Journal of Insects as Food and Feed, Vol. 4, No. 3, 2018, p. 179-186.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Campylobacter contamination level in houseflies after exposure to materials containing Campylobacter

AU - Nygaard Jensen, Annette

AU - Hald, Birthe

PY - 2018

Y1 - 2018

N2 - Although houseflies have been found to carry Campylobacter jejuni, little is known about the quantitative campylobacter level in naturally contaminated houseflies and their ability to contaminate surfaces. This study aimed to elucidate how houseflies’ previous exposure to campylobacter-contaminated material (faeces or liquid) for 1 or 4 h affects the acquired campylobacter level in houseflies and their contamination potential. Cups of 250 ml were added 5 g of chicken faeces or 1 ml liquid and spiked with approximately 3, 4, 5 or 7 log10 cfu C. jejuni. Sixteen houseflies were added to each cup. After 1 h of exposure, four houseflies were removed from the cup for enumeration of campylobacter in each fly by plate spreading. Another four houseflies were transferred onto Abeyta-Hunt-Bark (AHB) agar plates (9 cm) to assess possible contamination of surfaces. After 1 h on the AHB plate, each fly was tested for level of campylobacter. This procedure was repeated after approx. 4 h of exposure for the remaining eight houseflies. The C. jejuni acquisition in houseflies increased with exposure dose and was higher after liquid exposure compared with faeces exposure, while there was no significant effect of exposure time (1 vs 4 h). For faeces, 90.0% (n=80), 48.4% (n=64), 6.3% (n=48) and 0% (n=16) of houseflies were campylobacter-positive when exposed to 7, 5, 4, and 3 log10 cfu with a mean (±SE) of 2.0±0.1, 0.8±0.1, 0.3±0.0 and 0 log10 cfu recovered per campylobacter-positive fly, respectively. For liquid, 95.7% (n=47), 91.4% (n=47), 20.8% (n=48) and 6.3% (n=16) of houseflies were campylobacter-positive with a mean of 3.3±0.2, 2.0±0.1, 0.8±0.2 and 0.3±0.0 log10 cfu. The surface of the AHB plates was only contaminated by houseflies previously exposed to >4 log10, but the C. jejuni number found on the AHB surface did not correlate with the number found in the corresponding fly.

AB - Although houseflies have been found to carry Campylobacter jejuni, little is known about the quantitative campylobacter level in naturally contaminated houseflies and their ability to contaminate surfaces. This study aimed to elucidate how houseflies’ previous exposure to campylobacter-contaminated material (faeces or liquid) for 1 or 4 h affects the acquired campylobacter level in houseflies and their contamination potential. Cups of 250 ml were added 5 g of chicken faeces or 1 ml liquid and spiked with approximately 3, 4, 5 or 7 log10 cfu C. jejuni. Sixteen houseflies were added to each cup. After 1 h of exposure, four houseflies were removed from the cup for enumeration of campylobacter in each fly by plate spreading. Another four houseflies were transferred onto Abeyta-Hunt-Bark (AHB) agar plates (9 cm) to assess possible contamination of surfaces. After 1 h on the AHB plate, each fly was tested for level of campylobacter. This procedure was repeated after approx. 4 h of exposure for the remaining eight houseflies. The C. jejuni acquisition in houseflies increased with exposure dose and was higher after liquid exposure compared with faeces exposure, while there was no significant effect of exposure time (1 vs 4 h). For faeces, 90.0% (n=80), 48.4% (n=64), 6.3% (n=48) and 0% (n=16) of houseflies were campylobacter-positive when exposed to 7, 5, 4, and 3 log10 cfu with a mean (±SE) of 2.0±0.1, 0.8±0.1, 0.3±0.0 and 0 log10 cfu recovered per campylobacter-positive fly, respectively. For liquid, 95.7% (n=47), 91.4% (n=47), 20.8% (n=48) and 6.3% (n=16) of houseflies were campylobacter-positive with a mean of 3.3±0.2, 2.0±0.1, 0.8±0.2 and 0.3±0.0 log10 cfu. The surface of the AHB plates was only contaminated by houseflies previously exposed to >4 log10, but the C. jejuni number found on the AHB surface did not correlate with the number found in the corresponding fly.

KW - Houseflies

KW - Campylobacter transmission

KW - Exposure level

KW - Contamination

U2 - 10.3920/JIFF2018.0007

DO - 10.3920/JIFF2018.0007

M3 - Journal article

VL - 4

SP - 179

EP - 186

JO - Journal of Insects as Food and Feed

JF - Journal of Insects as Food and Feed

SN - 2352-4588

IS - 3

ER -