Calibration, standardization, and quantitative analysis of multidimensional fluorescence (MDF) measurements on complex mixtures (IUPAC Technical Report)

Alan G. Ryder, Colin Stedmon, Niels Harrit, Rasmus Bro

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Abstract

This IUPAC Technical Report describes and compares the currently applied methods for the calibration and standardization of multi-dimensional fluorescence (MDF) spectroscopy data as well as recommendations on the correct use of chemometric methods for MDF data analysis. The paper starts with a brief description of the measurement principles for the most important MDF techniques and a short introduction to the most important applications. Recommendations are provided for instrument calibration, sample preparation and handling, and data collection, as well as the proper use of chemometric data analysis methods.
Original languageEnglish
JournalPure and Applied Chemistry
Volume89
Issue number12
Pages (from-to)1849-1870
Number of pages22
ISSN0033-4545
DOIs
Publication statusPublished - 2017

Keywords

  • Calibration
  • Chemometrics
  • Excitation emission matric
  • Fluorescence
  • IUPAC

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