“Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images

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    Abstract

    In order to count photons with a camera, the camera must be calibrated. Photon counting is necessary, e.g., to determine the precision of localization-based super-resolution microscopy. Here we present a protocol that calibrates an EMCCD camera from information contained in isolated, diffraction-limited spots in any image taken by the camera, thus making dedicated calibration procedures redundant by enabling calibration post festum, from images filed without calibration information.
    Original languageEnglish
    Article number28680
    JournalScientific Reports
    Volume6
    Number of pages6
    ISSN2045-2322
    DOIs
    Publication statusPublished - 2016

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