Calibrated mitotic oscillator drives motile ciliogenesis

Adel Al Jord, Asm Shihavuddin, Raphaël Servignat D’Aout, Marion Faucourt, Auguste Genovesio, Anthi Karaiskou, Joëlle Sobczak-Thépot, Nathalie Spassky, Alice Meunier*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Cell division and differentiation depend on massive and rapid organelle remodeling. The mitotic oscillator, centered on the cyclin-dependent kinase 1–anaphase-promoting complex/cyclosome (CDK1-APC/C) axis, spatiotemporally coordinates this reorganization in dividing cells. Here we discovered that nondividing cells could also implement this mitotic clocklike regulatory circuit to orchestrate subcellular reorganization associated with differentiation. We probed centriole amplification in differentiating mouse-brain multiciliated cells. These postmitotic progenitors fine-tuned mitotic oscillator activity to drive the orderly progression of centriole production, maturation, and motile ciliation while avoiding the mitosis commitment threshold. Insufficient CDK1 activity hindered differentiation, whereas excessive activity accelerated differentiation yet drove postmitotic progenitors into mitosis. Thus, postmitotic cells can redeploy and calibrate the mitotic oscillator to uncouple cytoplasmic from nuclear dynamics for organelle remodeling associated with differentiation.

Original languageEnglish
JournalScience
Volume358
Issue number6364
Pages (from-to)803-806
Number of pages4
ISSN0036-8075
DOIs
Publication statusPublished - 10 Nov 2017
Externally publishedYes

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