Calcium-silicate-incorporated gellan-chitosan induced osteogenic differentiation in mesenchymal stromal cells

Krishnamurithy Genasan, Mohammad Mehrali, Tarini Veerappan, Sepehr Talebian, Murali Malliga Raman, Simmrat Singh, Sasikumar Swamiappan, Mehdi Mehrali, Tunku Kamarul*, Hanumantha Rao Balaji Raghavendran*

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) w/w was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS–seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (w/w) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects
    Original languageEnglish
    Article number3211
    JournalPolymers
    Volume13
    Issue number19
    Number of pages17
    ISSN2073-4360
    DOIs
    Publication statusPublished - 2021

    Bibliographical note

    This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

    Keywords

    • Bone
    • Bone tissue engineering
    • Calcium silicate
    • Chitosan
    • Gellan
    • Osteoinduction

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