Abstract
Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) w/w
was prepared using freeze-drying method to investigate its
physicochemical, biocompatible, and osteoinductive properties in human
bone-marrow mesenchymal stromal cells (hBMSCs). The composition of
different groups was reflected in physicochemical analyses performed
using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs
attachment in GC-40 CS. The Alamar Blue assay indicated an increased
proliferation and viability of seeded hBMSCs in all groups on day 21 as
compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic
differentiation based on an amplified alkaline-phosphatase release on
day 7 and 14 as compared with day 0. These cells supported bone
mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as
compared with day 7 and increased their osteogenic gene expression
(RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS–seeded
hBMSCs initiated their osteogenic differentiation on day 7 as compared
with counterparts based on an increased expression of type-1 collagen
and BMP2 in immunocytochemistry analysis. In conclusion, the
incorporation of 40% (w/w)
calcium silicate in gellan-chitosan showed osteoinduction potential in
hBMSCs, making it a potential biomaterial to treat critical bone defects
Original language | English |
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Article number | 3211 |
Journal | Polymers |
Volume | 13 |
Issue number | 19 |
Number of pages | 17 |
ISSN | 2073-4360 |
DOIs | |
Publication status | Published - 2021 |
Bibliographical note
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citedKeywords
- Bone
- Bone tissue engineering
- Calcium silicate
- Chitosan
- Gellan
- Osteoinduction