Calcium-silicate-incorporated gellan-chitosan induced osteogenic differentiation in mesenchymal stromal cells

Krishnamurithy Genasan; Mohammad Mehrali; Tarini Veerappan; Sepehr Talebian; Murali Malliga Raman; Simmrat Singh; Sasikumar Swamiappan; Mehdi Mehrali; Tunku Kamarul; Hanumantha Rao Balaji Raghavendran

doi:10.3390/polym13193211

Calcium-silicate-incorporated gellan-chitosan induced osteogenic differentiation in mesenchymal stromal cells

Krishnamurithy Genasan, Mohammad Mehrali, Tarini Veerappan, Sepehr Talebian, Murali Malliga Raman, Simmrat Singh, Sasikumar Swamiappan, Mehdi Mehrali, Tunku Kamarul^*, Hanumantha Rao Balaji Raghavendran^*

^*Corresponding author for this work

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Abstract

Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) w/w was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS–seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (w/w) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects

Original language	English
Article number	3211
Journal	Polymers
Volume	13
Issue number	19
Number of pages	17
ISSN	2073-4360
DOIs	https://doi.org/10.3390/polym13193211
Publication status	Published - 2021

Bibliographical note

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Keywords

Bone
Bone tissue engineering
Calcium silicate
Chitosan
Gellan
Osteoinduction

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Genasan, Krishnamurithy ; Mehrali, Mohammad ; Veerappan, Tarini ; Talebian, Sepehr ; Raman, Murali Malliga ; Singh, Simmrat ; Swamiappan, Sasikumar ; Mehrali, Mehdi ; Kamarul, Tunku ; Raghavendran, Hanumantha Rao Balaji. / Calcium-silicate-incorporated gellan-chitosan induced osteogenic differentiation in mesenchymal stromal cells. In: Polymers. 2021 ; Vol. 13, No. 19.

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title = "Calcium-silicate-incorporated gellan-chitosan induced osteogenic differentiation in mesenchymal stromal cells",

abstract = "Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) w/w was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS–seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (w/w) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects",

keywords = "Bone, Bone tissue engineering, Calcium silicate, Chitosan, Gellan, Osteoinduction",

author = "Krishnamurithy Genasan and Mohammad Mehrali and Tarini Veerappan and Sepehr Talebian and Raman, {Murali Malliga} and Simmrat Singh and Sasikumar Swamiappan and Mehdi Mehrali and Tunku Kamarul and Raghavendran, {Hanumantha Rao Balaji}",

note = "This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited ",

year = "2021",

doi = "10.3390/polym13193211",

language = "English",

volume = "13",

journal = "Polymers",

issn = "2073-4360",

publisher = "M D P I AG",

number = "19",

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Calcium-silicate-incorporated gellan-chitosan induced osteogenic differentiation in mesenchymal stromal cells. / Genasan, Krishnamurithy; Mehrali, Mohammad; Veerappan, Tarini; Talebian, Sepehr; Raman, Murali Malliga; Singh, Simmrat; Swamiappan, Sasikumar; Mehrali, Mehdi; Kamarul, Tunku; Raghavendran, Hanumantha Rao Balaji.

In: Polymers, Vol. 13, No. 19, 3211, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Calcium-silicate-incorporated gellan-chitosan induced osteogenic differentiation in mesenchymal stromal cells

AU - Genasan, Krishnamurithy

AU - Mehrali, Mohammad

AU - Veerappan, Tarini

AU - Talebian, Sepehr

AU - Raman, Murali Malliga

AU - Singh, Simmrat

AU - Swamiappan, Sasikumar

AU - Mehrali, Mehdi

AU - Kamarul, Tunku

AU - Raghavendran, Hanumantha Rao Balaji

N1 - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

PY - 2021

Y1 - 2021

N2 - Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) w/w was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS–seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (w/w) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects

AB - Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) w/w was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS–seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (w/w) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects

KW - Bone

KW - Bone tissue engineering

KW - Calcium silicate

KW - Chitosan

KW - Gellan

KW - Osteoinduction

U2 - 10.3390/polym13193211

DO - 10.3390/polym13193211

M3 - Journal article

VL - 13

JO - Polymers

JF - Polymers

SN - 2073-4360

IS - 19

M1 - 3211

ER -

Calcium-silicate-incorporated gellan-chitosan induced osteogenic differentiation in mesenchymal stromal cells

Abstract

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