Butyrate production in engineered Escherichia coli with synthetic scaffolds

Jang-Mi Baek, Suman Mazumdar, Sang-Woo Lee, Moo-Young Jung, Jae-Hyung Lim, Sang Woo Seo, Gyoo-Yeol Jung, Min-Kyu Oh

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Butyrate pathway was constructed in recombinant Escherichia coli using the genes from Clostridium acetobutylicum and Treponema denticola. However, the pathway constructed from exogenous enzymes did not efficiently convert carbon flux to butyrate. Three steps of the productivity enhancement were attempted in this study. First, pathway engineering to delete metabolic pathways to by-products successfully improved the butyrate production. Second, synthetic scaffold protein that spatially co-localizes enzymes was introduced to improve the efficiency of the heterologous pathway enzymes, resulting in threefold improvement in butyrate production. Finally, further optimizations of inducer concentrations and pH adjustment were tried. The final titer of butyrate was 4.3 and 7.2 g/Lunder batch and fed-batch cultivation, respectively. This study demonstrated the importance of synthetic scaffold protein as a useful tool for optimization of heterologous butyrate pathway in E. coli.
Original languageEnglish
JournalBiotechnology and Bioengineering (Print)
Volume110
Issue number10
Pages (from-to)2790–2794
ISSN0006-3592
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Escherichia coli
  • Butyrate
  • Synthetic scaffold
  • Metabolic engineering
  • Heterologous pathway

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