Aortic vascular prosthetic graft infection (AVPGI) with Staphylococcus aureus is a feared post-operative complication. This study was conducted to evaluate the clinical signs and potential biomarkers of infection in a porcine AVPGI model. The biomarkers evaluated were: C-reactive protein (CRP), fibrinogen, white blood cells (WBC), major histocompatibility complex II (MHC II) density, lymphocyte CD4:CD8 ratio and tumour necrosis factor-alpha (TNF-α) in vitro responsiveness. Sixteen pigs were included in the study, and randomly assigned into four groups (n = 4): “SHAM” pigs had their infra-renal aorta exposed by laparotomy; “CLEAN” pigs had an aortic graft inserted; “LOW” and “HIGH” pigs had an aortic graft inserted and, subsequently, S. aureus were inoculated on the graft material (5 × 104 colony-forming units [CFU] and 1 × 106 CFU, respectively). Biomarkers were evaluated prior to surgery and on day 2, 5, 7, and 14 post-operatively in blood samples. Of all biomarkers evaluated, CRP was superior for diagnosing S. aureus AVPGI in pigs, with a sensitivity of 0.86 and a specificity of 0.75.
|Journal||European Journal of Clinical Microbiology & Infectious Diseases|
|Publication status||Published - 2010|