Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius

Fernanda Pelisson Massi; Maria Lucia Cameiro Vieira; Daniele Sartori; Rafael Elias Silva Penha; Carla de Freitas Munhoz; Josue Maldonado Ferreira; Beatriz Thie Iamanaka; Marta Hiromi Taniwaki; Jens Christian Frisvad; Maria Helena Pelegrinelli Fungaro

doi:10.1016/j.ijfoodmicro.2014.06.006

Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius

Fernanda Pelisson Massi, Maria Lucia Cameiro Vieira, Daniele Sartori, Rafael Elias Silva Penha, Carla de Freitas Munhoz, Josue Maldonado Ferreira, Beatriz Thie Iamanaka, Marta Hiromi Taniwaki, Jens Christian Frisvad, Maria Helena Pelegrinelli Fungaro

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The exploitation of the Brazil nut is one of the most important activities of the extractive communities of the Amazon rainforest. However, its commercialization can be affected by the presence of aflatoxins produced by fungi, namely Aspergillus section Flavi. In the present study, we investigated a collection of Aspergillus nomius strains isolated from Brazil nuts using different approaches, including morphological characters, RAPD and AFLP profiles, partial beta-tubulin and calmodulin nucleotide sequences, aflatoxin patterns, as well as tolerance to low water activity in cultured media. Results showed that most of the isolates do belong to A. nomius species, but a few were re-identified as Aspergillus pseudonomius, a very recently described species. The results of the analyses of molecular variance, as well as the high pairwise F-ST values between A. nomius and A. pseudonomius suggested the isolation between these two species and the inexistence of gene flow. Fixed interspecific nucleotide polymorphisms at beta-tubulin and calmodulin loci are presented. All A. pseudonomius strains analyzed produced aflatoxins AFB1, AFB2, AFG1 and AFG2. This study contains the first-ever report on the occurrence in Brazil nuts of A. pseudonomius. The G-type aflatoxins and the mycotoxin tenuazonic acid are reported here for the first time in A. pseudonomius.

Original language	English
Journal	International Journal of Food Microbiology
Volume	186
Pages (from-to)	14-21
Number of pages	8
ISSN	0168-1605
DOIs	https://doi.org/10.1016/j.ijfoodmicro.2014.06.006
Publication status	Published - 2014

Keywords

Amazon Rain Forest South America Neotropical region
Dicotyledones Angiospermae Spermatophyta Plantae (Angiosperms, Dicots, Plants, Spermatophytes, Vascular Plants) - Lecythidaceae [26250] Bertholletia excelsa species Brazil nut common host
Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes [15500] Aspergillus nomius species pathogen Aspergillus pseudonomius species pathogen
Aspergillus pseudonomius benA gene [Fungi Imperfecti or Deuteromycetes] polymorphism
Aspergillus pseudonomius cmd gene [Fungi Imperfecti or Deuteromycetes] polymorphism
aflatoxin B-1 1162-65-8 synthesis
aflatoxin B2 7220-81-7
aflatoxin G-1 1165-39-5
aflatoxin G2 7241-98-7
beta-tubulin
calmodulin
tenuazonic acid 75652-74-3 toxin, mycotoxin
03502, Genetics - General
03504, Genetics - Plant
10060, Biochemistry studies - General
10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
10064, Biochemistry studies - Proteins, peptides and amino acids
13502, Food technology - General and methods
13504, Food technology - Fruits, nuts and vegetables
22501, Toxicology - General and methods
22502, Toxicology - Foods, food residues, additives and preservatives
36001, Medical and clinical microbiology - General and methods
Foods
Infection
Molecular Genetics
Toxicology
Brazil nut
infection Infection (MeSH) infectious disease etiology
Biochemistry and Molecular Biophysics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijfoodmicro.2014.06.006

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Massi, F. P., Cameiro Vieira, M. L., Sartori, D., Silva Penha, R. E., Munhoz, C. D. F., Ferreira, J. M., Iamanaka, B. T., Taniwaki, M. H., Frisvad, J. C., & Pelegrinelli Fungaro, M. H. (2014). Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius. International Journal of Food Microbiology, 186, 14-21. https://doi.org/10.1016/j.ijfoodmicro.2014.06.006

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abstract = "The exploitation of the Brazil nut is one of the most important activities of the extractive communities of the Amazon rainforest. However, its commercialization can be affected by the presence of aflatoxins produced by fungi, namely Aspergillus section Flavi. In the present study, we investigated a collection of Aspergillus nomius strains isolated from Brazil nuts using different approaches, including morphological characters, RAPD and AFLP profiles, partial beta-tubulin and calmodulin nucleotide sequences, aflatoxin patterns, as well as tolerance to low water activity in cultured media. Results showed that most of the isolates do belong to A. nomius species, but a few were re-identified as Aspergillus pseudonomius, a very recently described species. The results of the analyses of molecular variance, as well as the high pairwise F-ST values between A. nomius and A. pseudonomius suggested the isolation between these two species and the inexistence of gene flow. Fixed interspecific nucleotide polymorphisms at beta-tubulin and calmodulin loci are presented. All A. pseudonomius strains analyzed produced aflatoxins AFB1, AFB2, AFG1 and AFG2. This study contains the first-ever report on the occurrence in Brazil nuts of A. pseudonomius. The G-type aflatoxins and the mycotoxin tenuazonic acid are reported here for the first time in A. pseudonomius. ",

keywords = "Amazon Rain Forest South America Neotropical region, Dicotyledones Angiospermae Spermatophyta Plantae (Angiosperms, Dicots, Plants, Spermatophytes, Vascular Plants) - Lecythidaceae [26250] Bertholletia excelsa species Brazil nut common host, Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes [15500] Aspergillus nomius species pathogen Aspergillus pseudonomius species pathogen, Aspergillus pseudonomius benA gene [Fungi Imperfecti or Deuteromycetes] polymorphism, Aspergillus pseudonomius cmd gene [Fungi Imperfecti or Deuteromycetes] polymorphism, aflatoxin B-1 1162-65-8 synthesis, aflatoxin B2 7220-81-7, aflatoxin G-1 1165-39-5, aflatoxin G2 7241-98-7, beta-tubulin, calmodulin, tenuazonic acid 75652-74-3 toxin, mycotoxin, 03502, Genetics - General, 03504, Genetics - Plant, 10060, Biochemistry studies - General, 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines, 10064, Biochemistry studies - Proteins, peptides and amino acids, 13502, Food technology - General and methods, 13504, Food technology - Fruits, nuts and vegetables, 22501, Toxicology - General and methods, 22502, Toxicology - Foods, food residues, additives and preservatives, 36001, Medical and clinical microbiology - General and methods, Foods, Infection, Molecular Genetics, Toxicology, Brazil nut, infection Infection (MeSH) infectious disease etiology, Biochemistry and Molecular Biophysics",

author = "Massi, {Fernanda Pelisson} and {Cameiro Vieira}, {Maria Lucia} and Daniele Sartori and {Silva Penha}, {Rafael Elias} and Munhoz, {Carla de Freitas} and Ferreira, {Josue Maldonado} and Iamanaka, {Beatriz Thie} and Taniwaki, {Marta Hiromi} and Frisvad, {Jens Christian} and {Pelegrinelli Fungaro}, {Maria Helena}",

year = "2014",

doi = "10.1016/j.ijfoodmicro.2014.06.006",

language = "English",

volume = "186",

pages = "14--21",

journal = "International Journal of Food Microbiology",

issn = "0168-1605",

publisher = "Elsevier",

}

Massi, FP, Cameiro Vieira, ML, Sartori, D, Silva Penha, RE, Munhoz, CDF, Ferreira, JM, Iamanaka, BT, Taniwaki, MH, Frisvad, JC & Pelegrinelli Fungaro, MH 2014, 'Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius', International Journal of Food Microbiology, vol. 186, pp. 14-21. https://doi.org/10.1016/j.ijfoodmicro.2014.06.006

TY - JOUR

T1 - Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius

AU - Massi, Fernanda Pelisson

AU - Cameiro Vieira, Maria Lucia

AU - Sartori, Daniele

AU - Silva Penha, Rafael Elias

AU - Munhoz, Carla de Freitas

AU - Ferreira, Josue Maldonado

AU - Iamanaka, Beatriz Thie

AU - Taniwaki, Marta Hiromi

AU - Frisvad, Jens Christian

AU - Pelegrinelli Fungaro, Maria Helena

PY - 2014

Y1 - 2014

N2 - The exploitation of the Brazil nut is one of the most important activities of the extractive communities of the Amazon rainforest. However, its commercialization can be affected by the presence of aflatoxins produced by fungi, namely Aspergillus section Flavi. In the present study, we investigated a collection of Aspergillus nomius strains isolated from Brazil nuts using different approaches, including morphological characters, RAPD and AFLP profiles, partial beta-tubulin and calmodulin nucleotide sequences, aflatoxin patterns, as well as tolerance to low water activity in cultured media. Results showed that most of the isolates do belong to A. nomius species, but a few were re-identified as Aspergillus pseudonomius, a very recently described species. The results of the analyses of molecular variance, as well as the high pairwise F-ST values between A. nomius and A. pseudonomius suggested the isolation between these two species and the inexistence of gene flow. Fixed interspecific nucleotide polymorphisms at beta-tubulin and calmodulin loci are presented. All A. pseudonomius strains analyzed produced aflatoxins AFB1, AFB2, AFG1 and AFG2. This study contains the first-ever report on the occurrence in Brazil nuts of A. pseudonomius. The G-type aflatoxins and the mycotoxin tenuazonic acid are reported here for the first time in A. pseudonomius.

AB - The exploitation of the Brazil nut is one of the most important activities of the extractive communities of the Amazon rainforest. However, its commercialization can be affected by the presence of aflatoxins produced by fungi, namely Aspergillus section Flavi. In the present study, we investigated a collection of Aspergillus nomius strains isolated from Brazil nuts using different approaches, including morphological characters, RAPD and AFLP profiles, partial beta-tubulin and calmodulin nucleotide sequences, aflatoxin patterns, as well as tolerance to low water activity in cultured media. Results showed that most of the isolates do belong to A. nomius species, but a few were re-identified as Aspergillus pseudonomius, a very recently described species. The results of the analyses of molecular variance, as well as the high pairwise F-ST values between A. nomius and A. pseudonomius suggested the isolation between these two species and the inexistence of gene flow. Fixed interspecific nucleotide polymorphisms at beta-tubulin and calmodulin loci are presented. All A. pseudonomius strains analyzed produced aflatoxins AFB1, AFB2, AFG1 and AFG2. This study contains the first-ever report on the occurrence in Brazil nuts of A. pseudonomius. The G-type aflatoxins and the mycotoxin tenuazonic acid are reported here for the first time in A. pseudonomius.

KW - Amazon Rain Forest South America Neotropical region

KW - Dicotyledones Angiospermae Spermatophyta Plantae (Angiosperms, Dicots, Plants, Spermatophytes, Vascular Plants) - Lecythidaceae [26250] Bertholletia excelsa species Brazil nut common host

KW - Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes [15500] Aspergillus nomius species pathogen Aspergillus pseudonomius species pathogen

KW - Aspergillus pseudonomius benA gene [Fungi Imperfecti or Deuteromycetes] polymorphism

KW - Aspergillus pseudonomius cmd gene [Fungi Imperfecti or Deuteromycetes] polymorphism

KW - aflatoxin B-1 1162-65-8 synthesis

KW - aflatoxin B2 7220-81-7

KW - aflatoxin G-1 1165-39-5

KW - aflatoxin G2 7241-98-7

KW - beta-tubulin

KW - calmodulin

KW - tenuazonic acid 75652-74-3 toxin, mycotoxin

KW - 03502, Genetics - General

KW - 03504, Genetics - Plant

KW - 10060, Biochemistry studies - General

KW - 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines

KW - 10064, Biochemistry studies - Proteins, peptides and amino acids

KW - 13502, Food technology - General and methods

KW - 13504, Food technology - Fruits, nuts and vegetables

KW - 22501, Toxicology - General and methods

KW - 22502, Toxicology - Foods, food residues, additives and preservatives

KW - 36001, Medical and clinical microbiology - General and methods

KW - Foods

KW - Infection

KW - Molecular Genetics

KW - Toxicology

KW - Brazil nut

KW - infection Infection (MeSH) infectious disease etiology

KW - Biochemistry and Molecular Biophysics

U2 - 10.1016/j.ijfoodmicro.2014.06.006

DO - 10.1016/j.ijfoodmicro.2014.06.006

M3 - Journal article

C2 - 24974275

SN - 0168-1605

VL - 186

SP - 14

EP - 21

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

ER -

Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius

Abstract

Keywords

UN SDGs

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