Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 6 in pig serum

Joan Klausen; Lars Ole Andresen; Kristen Barfod; Vibeke Sørensen

doi:10.1016/S0378-1135(00)00349-7

Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 6 in pig serum

Joan Klausen, Lars Ole Andresen, Kristen Barfod, Vibeke Sørensen

National Food Institute

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A blocking enzyme-linked immunosorbent assay (ELISA) detecting antibodies against Actinobacillus pleuropneumoniae (Ap) serotype 6 was developed. The blocking ELISA was based on the inhibition of a polyclonal antibody raised against Ap serotype 6. Purified lipopolysaccharide from Ap serotype 6 was used as antigen. The blocking ELISA was tested against sera from pigs experimentally infected with the 12 serotypes of Ap biotype 1. Cross-reaction with serotypes 3 and 8 but not with other serotypes was observed. The sensitivity and specificity of the test on a herd level were evaluated with sera from herds naturally infected with serotypes 2, 6, 8 or 12 and with sera from herds free of infection with any Ap serotype. The blocking ELISA showed a high herd sensitivity (1.00 (0.79-1.00)) and specificity (0.97 (0.93-0.99)).

Original language	English
Journal	Veterinary Microbiology
Volume	79
Issue number	1
Pages (from-to)	11-18
ISSN	0378-1135
DOIs	https://doi.org/10.1016/S0378-1135(00)00349-7
Publication status	Published - 2001

Keywords

serological assay
Actinobacillus pleuropneumoniae
pig-bacteria
ELISA
lipopolysaccharide
pleuropneumonia

Access to Document

10.1016/S0378-1135(00)00349-7

OpenUrl availability

Full text

Cite this

@article{0a7aac04944b4d5b87680f5ac2893e17,

title = "Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 6 in pig serum",

abstract = "A blocking enzyme-linked immunosorbent assay (ELISA) detecting antibodies against Actinobacillus pleuropneumoniae (Ap) serotype 6 was developed. The blocking ELISA was based on the inhibition of a polyclonal antibody raised against Ap serotype 6. Purified lipopolysaccharide from Ap serotype 6 was used as antigen. The blocking ELISA was tested against sera from pigs experimentally infected with the 12 serotypes of Ap biotype 1. Cross-reaction with serotypes 3 and 8 but not with other serotypes was observed. The sensitivity and specificity of the test on a herd level were evaluated with sera from herds naturally infected with serotypes 2, 6, 8 or 12 and with sera from herds free of infection with any Ap serotype. The blocking ELISA showed a high herd sensitivity (1.00 (0.79-1.00)) and specificity (0.97 (0.93-0.99)).",

keywords = "serological assay, Actinobacillus pleuropneumoniae, pig-bacteria, ELISA, lipopolysaccharide, pleuropneumonia",

author = "Joan Klausen and Andresen, {Lars Ole} and Kristen Barfod and Vibeke S{\o}rensen",

year = "2001",

doi = "10.1016/S0378-1135(00)00349-7",

language = "English",

volume = "79",

pages = "11--18",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 6 in pig serum

AU - Klausen, Joan

AU - Andresen, Lars Ole

AU - Barfod, Kristen

AU - Sørensen, Vibeke

PY - 2001

Y1 - 2001

N2 - A blocking enzyme-linked immunosorbent assay (ELISA) detecting antibodies against Actinobacillus pleuropneumoniae (Ap) serotype 6 was developed. The blocking ELISA was based on the inhibition of a polyclonal antibody raised against Ap serotype 6. Purified lipopolysaccharide from Ap serotype 6 was used as antigen. The blocking ELISA was tested against sera from pigs experimentally infected with the 12 serotypes of Ap biotype 1. Cross-reaction with serotypes 3 and 8 but not with other serotypes was observed. The sensitivity and specificity of the test on a herd level were evaluated with sera from herds naturally infected with serotypes 2, 6, 8 or 12 and with sera from herds free of infection with any Ap serotype. The blocking ELISA showed a high herd sensitivity (1.00 (0.79-1.00)) and specificity (0.97 (0.93-0.99)).

AB - A blocking enzyme-linked immunosorbent assay (ELISA) detecting antibodies against Actinobacillus pleuropneumoniae (Ap) serotype 6 was developed. The blocking ELISA was based on the inhibition of a polyclonal antibody raised against Ap serotype 6. Purified lipopolysaccharide from Ap serotype 6 was used as antigen. The blocking ELISA was tested against sera from pigs experimentally infected with the 12 serotypes of Ap biotype 1. Cross-reaction with serotypes 3 and 8 but not with other serotypes was observed. The sensitivity and specificity of the test on a herd level were evaluated with sera from herds naturally infected with serotypes 2, 6, 8 or 12 and with sera from herds free of infection with any Ap serotype. The blocking ELISA showed a high herd sensitivity (1.00 (0.79-1.00)) and specificity (0.97 (0.93-0.99)).

KW - serological assay

KW - Actinobacillus pleuropneumoniae

KW - pig-bacteria

KW - ELISA

KW - lipopolysaccharide

KW - pleuropneumonia

U2 - 10.1016/S0378-1135(00)00349-7

DO - 10.1016/S0378-1135(00)00349-7

M3 - Journal article

SN - 0378-1135

VL - 79

SP - 11

EP - 18

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 1

ER -

Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 6 in pig serum

Abstract

Keywords

Access to Document

OpenUrl availability

Fingerprint

Cite this