Blocking ELISA's for the distinction between antibodies against European and American strains of porcine reproductive and respiratory syndrome virus

K Sørensen, Bertel Strandbygaard, Anette Bøtner, E.S. Madsen, Jens Nielsen, P. Have

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A double blocking ELISA was developed in order to satisfy the need for large scale serological screening for PRRS and simultaneous distinction between infection with European and American strains of PRRSV in pig herds. The Immunoperoxidase monolayer assay (IPMA) and the double blocking ELISA enabled distinction on serological basis between infection with European and American strains of PRRSV. The distinction was possible from about day 7 after infection of pigs with PRRSV. The double blocking ELISA enabled the distinction at later stages of infection compared to the IPMA, irrespective of the strain involved.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume60
    Issue number2-4
    Pages (from-to)169-177
    ISSN0378-1135
    Publication statusPublished - 1998

    Keywords

    • PRRS
    • American strain
    • antibodies
    • European strain
    • ELISA

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