Bladder wall biomechanics: A comprehensive study on fresh porcine urinary bladder

Maryam Sami Jokandan, Fatemeh Ajalloueian*, Magnus Edinger, Peter Reimer Stubbe, Stefania Baldursdottir, Ioannis S. Chronakis

*Corresponding author for this work

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Abstract

Regenerative medicine for reconstructive urogenital surgery has been widely studied during the last two decades. One of the key factors affecting the quality of bladder regeneration is the mechanical properties of the bladder scaffold. Insight into the biomechanics of this organ is expected to assist researchers with functional regeneration of the bladder wall. Due to extensive similarities between human bladder and porcine bladder, and with regard to lack of comprehensive biomechanical data from the porcine bladder wall (BW), our main goal here was to provide a thorough evaluation on viscoelastic properties of fresh porcine urinary BW. Three testing modes including Uniaxial tensile, Ball-burst (BB) and Dynamic Mechanical Analyses (DMA) were applied in parallel. Uniaxial tests were applied to study how different circumferential and longitudinal cut-outs of lateral region of BW behave under load. DMA was used to measure the viscoelastic properties of the bladder tissue (storage and loss modulus) tested in a frequency range of 0.1 to 3 Hz. BB was selected as a different technique replicating normal physiological conditions where the BW is studied in whole. According to uniaxial tests, the anisotropic behavior of bladder was evident at strain loads higher than 200%. According to DMA, storage modulus was found to be consistently higher than loss modulus in both directions, revealing the elasticity of the BW. The stress-strain curves of both uniaxial and BB tests showed similar trends. However, the ultimate stress measured from BB was found to be around 5 times of the relevant stress from uniaxial loading. The ultimate strain in BB (389.9 ± 59.8) was interestingly an approximate average of longitudinal (358 ± 21) and circumferential (435 ± 69) rupture strains. Considering that each testing mode applied here reveals distinct information, outcomes from the combination of the three can be considered as a helpful data-base to refer to for researchers aiming to regenerate the bladder.
Original languageEnglish
JournalJournal of the Mechanical Behavior of Biomedical Materials
Volume79
Pages (from-to)92-103
ISSN1751-6161
DOIs
Publication statusPublished - 2018

Cite this

@article{e68a31cb9ef7451da8318fbf28c5e800,
title = "Bladder wall biomechanics: A comprehensive study on fresh porcine urinary bladder",
abstract = "Regenerative medicine for reconstructive urogenital surgery has been widely studied during the last two decades. One of the key factors affecting the quality of bladder regeneration is the mechanical properties of the bladder scaffold. Insight into the biomechanics of this organ is expected to assist researchers with functional regeneration of the bladder wall. Due to extensive similarities between human bladder and porcine bladder, and with regard to lack of comprehensive biomechanical data from the porcine bladder wall (BW), our main goal here was to provide a thorough evaluation on viscoelastic properties of fresh porcine urinary BW. Three testing modes including Uniaxial tensile, Ball-burst (BB) and Dynamic Mechanical Analyses (DMA) were applied in parallel. Uniaxial tests were applied to study how different circumferential and longitudinal cut-outs of lateral region of BW behave under load. DMA was used to measure the viscoelastic properties of the bladder tissue (storage and loss modulus) tested in a frequency range of 0.1 to 3 Hz. BB was selected as a different technique replicating normal physiological conditions where the BW is studied in whole. According to uniaxial tests, the anisotropic behavior of bladder was evident at strain loads higher than 200{\%}. According to DMA, storage modulus was found to be consistently higher than loss modulus in both directions, revealing the elasticity of the BW. The stress-strain curves of both uniaxial and BB tests showed similar trends. However, the ultimate stress measured from BB was found to be around 5 times of the relevant stress from uniaxial loading. The ultimate strain in BB (389.9 ± 59.8) was interestingly an approximate average of longitudinal (358 ± 21) and circumferential (435 ± 69) rupture strains. Considering that each testing mode applied here reveals distinct information, outcomes from the combination of the three can be considered as a helpful data-base to refer to for researchers aiming to regenerate the bladder.",
author = "{Sami Jokandan}, Maryam and Fatemeh Ajalloueian and Magnus Edinger and Stubbe, {Peter Reimer} and Stefania Baldursdottir and Chronakis, {Ioannis S.}",
year = "2018",
doi = "10.1016/j.jmbbm.2017.11.034",
language = "English",
volume = "79",
pages = "92--103",
journal = "Journal of the Mechanical Behavior of Biomedical Materials",
issn = "1751-6161",
publisher = "Elsevier",

}

Bladder wall biomechanics: A comprehensive study on fresh porcine urinary bladder. / Sami Jokandan, Maryam; Ajalloueian, Fatemeh; Edinger, Magnus; Stubbe, Peter Reimer; Baldursdottir, Stefania; Chronakis, Ioannis S.

In: Journal of the Mechanical Behavior of Biomedical Materials, Vol. 79, 2018, p. 92-103.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Bladder wall biomechanics: A comprehensive study on fresh porcine urinary bladder

AU - Sami Jokandan, Maryam

AU - Ajalloueian, Fatemeh

AU - Edinger, Magnus

AU - Stubbe, Peter Reimer

AU - Baldursdottir, Stefania

AU - Chronakis, Ioannis S.

PY - 2018

Y1 - 2018

N2 - Regenerative medicine for reconstructive urogenital surgery has been widely studied during the last two decades. One of the key factors affecting the quality of bladder regeneration is the mechanical properties of the bladder scaffold. Insight into the biomechanics of this organ is expected to assist researchers with functional regeneration of the bladder wall. Due to extensive similarities between human bladder and porcine bladder, and with regard to lack of comprehensive biomechanical data from the porcine bladder wall (BW), our main goal here was to provide a thorough evaluation on viscoelastic properties of fresh porcine urinary BW. Three testing modes including Uniaxial tensile, Ball-burst (BB) and Dynamic Mechanical Analyses (DMA) were applied in parallel. Uniaxial tests were applied to study how different circumferential and longitudinal cut-outs of lateral region of BW behave under load. DMA was used to measure the viscoelastic properties of the bladder tissue (storage and loss modulus) tested in a frequency range of 0.1 to 3 Hz. BB was selected as a different technique replicating normal physiological conditions where the BW is studied in whole. According to uniaxial tests, the anisotropic behavior of bladder was evident at strain loads higher than 200%. According to DMA, storage modulus was found to be consistently higher than loss modulus in both directions, revealing the elasticity of the BW. The stress-strain curves of both uniaxial and BB tests showed similar trends. However, the ultimate stress measured from BB was found to be around 5 times of the relevant stress from uniaxial loading. The ultimate strain in BB (389.9 ± 59.8) was interestingly an approximate average of longitudinal (358 ± 21) and circumferential (435 ± 69) rupture strains. Considering that each testing mode applied here reveals distinct information, outcomes from the combination of the three can be considered as a helpful data-base to refer to for researchers aiming to regenerate the bladder.

AB - Regenerative medicine for reconstructive urogenital surgery has been widely studied during the last two decades. One of the key factors affecting the quality of bladder regeneration is the mechanical properties of the bladder scaffold. Insight into the biomechanics of this organ is expected to assist researchers with functional regeneration of the bladder wall. Due to extensive similarities between human bladder and porcine bladder, and with regard to lack of comprehensive biomechanical data from the porcine bladder wall (BW), our main goal here was to provide a thorough evaluation on viscoelastic properties of fresh porcine urinary BW. Three testing modes including Uniaxial tensile, Ball-burst (BB) and Dynamic Mechanical Analyses (DMA) were applied in parallel. Uniaxial tests were applied to study how different circumferential and longitudinal cut-outs of lateral region of BW behave under load. DMA was used to measure the viscoelastic properties of the bladder tissue (storage and loss modulus) tested in a frequency range of 0.1 to 3 Hz. BB was selected as a different technique replicating normal physiological conditions where the BW is studied in whole. According to uniaxial tests, the anisotropic behavior of bladder was evident at strain loads higher than 200%. According to DMA, storage modulus was found to be consistently higher than loss modulus in both directions, revealing the elasticity of the BW. The stress-strain curves of both uniaxial and BB tests showed similar trends. However, the ultimate stress measured from BB was found to be around 5 times of the relevant stress from uniaxial loading. The ultimate strain in BB (389.9 ± 59.8) was interestingly an approximate average of longitudinal (358 ± 21) and circumferential (435 ± 69) rupture strains. Considering that each testing mode applied here reveals distinct information, outcomes from the combination of the three can be considered as a helpful data-base to refer to for researchers aiming to regenerate the bladder.

U2 - 10.1016/j.jmbbm.2017.11.034

DO - 10.1016/j.jmbbm.2017.11.034

M3 - Journal article

VL - 79

SP - 92

EP - 103

JO - Journal of the Mechanical Behavior of Biomedical Materials

JF - Journal of the Mechanical Behavior of Biomedical Materials

SN - 1751-6161

ER -