Black perithecial pigmentation in Fusarium species is due to the accumulation of 5-deoxybostrycoidin-based melanin

Rasmus John Normand Frandsen, Silas Anselm Rasmussen, Peter Boldsen Knudsen, Silvio Uhlig, Dirk Petersen, Erik Lysøe, Charlotte Held Gotfredsen, Henriette Giese, Thomas Ostenfeld Larsen

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Abstract

Biosynthesis of the black perithecial pigment in the filamentous fungus Fusarium graminearum is dependent on the polyketide synthase PGL1 (oPKS3). A seven-membered PGL1 gene cluster was identified by over-expression of the cluster specific transcription factor pglR. Targeted gene replacement showed that PGL1, pglJ, pglM and pglV were essential for the production of the perithecial pigment. Over-expression of PGL1 resulted in the production of 6-O-demethyl-5-deoxybostrycoidin (1), 5-deoxybostrycoidin (2), and three novel compounds 5-deoxybostrycoidin anthrone (3), 6-O-demethyl-5-deoxybostrycoidin anthrone (4) and purpurfusarin (5). The novel dimeric bostrycoidin purpurfusarin (5) was found to inhibit the growth of Candida albicans with an IC50 of 8.0 +/-1.9 mu M. The results show that Fusarium species with black perithecia have a previously undescribed form of 5-deoxybostrycoidin based melanin in their fruiting bodies.
Original languageEnglish
Article number26206
JournalScientific Reports
Volume6
Number of pages13
ISSN2045-2322
DOIs
Publication statusPublished - 2016

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Keywords

  • Fungal biology
  • Metabolomics
  • Molecular evolution

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