Biotransformation of the citrus flavone tangeretin in rats. Identification of metabolites with intact flavane nucleus

S.E. Nielsen, V. Breinholt, C. Cornett, L.O. Dragsted

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The present study mas carried out in order to investigate the in vivo biotransformation and excretion of the flavone, tangeretin, found in citrus fruits, by analysing urine and faeces samples from rats after repeated administration of 100 mg/kg body weight/day tangeretin. The formed metabolites were separated and identified by HPLC and the structures elucidated by LC/MS and H-1 NMR. Ten new, major metabolites with intact flavonoid structure were identified. The metabolites identified were either demethylated or hydroxylated derivatives of the parent compound and metabolic changes were found primarily to occur in the 4' position of the B-ring. The total urinary excretion of tangeretin metabolites with intact flavan nucleus sas about 11% of the administered daily dose.
Original languageEnglish
JournalFood and Chemical Toxicology
Volume38
Issue number9
Pages (from-to)739-746
ISSN0278-6915
DOIs
Publication statusPublished - 2000

Keywords

  • metabolism HPLC
  • citrus flavone
  • rat
  • tangeretin

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