Biotin Carboxyl Carrier Protein in Barley Chloroplast Membranes

C. G. Kannangara, C J Jense

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Biotin localized in barley chloroplast lamellae is covalently bound to a single protein with an approximate molecular weight of 21000. It contains one mole of biotin per mole of protein and functions as a carboxyl carrier in the acetyl-CoA carboxylase reaction. The protein was obtained by solubilization of the lamellae in phenol/acetic acid/8 M urea. Feeding barley seedlings with [14C]-biotin revealed that the vitamin is not degraded into respiratory substrates by the plant, but is specifically incorporated into biotin carboxyl carrier protein.
    Original languageEnglish
    JournalEuropean Journal of Biochemistry
    Volume54
    Issue number1
    Pages (from-to)25-30
    ISSN0014-2956
    DOIs
    Publication statusPublished - 1975

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