Biotin localized in barley chloroplast lamellae is covalently bound to a single protein with an approximate molecular weight of 21000. It contains one mole of biotin per mole of protein and functions as a carboxyl carrier in the acetyl-CoA carboxylase reaction. The protein was obtained by solubilization of the lamellae in phenol/acetic acid/8 M urea. Feeding barley seedlings with [14C]-biotin revealed that the vitamin is not degraded into respiratory substrates by the plant, but is specifically incorporated into biotin carboxyl carrier protein.
|Journal||European Journal of Biochemistry|
|Publication status||Published - 1975|
Kannangara, C. G., & Jense, C. J. (1975). Biotin Carboxyl Carrier Protein in Barley Chloroplast Membranes. European Journal of Biochemistry, 54(1), 25-30. https://doi.org/10.1111/j.1432-1033.1975.tb04109.x