Biosynthetic Code for Divergolide Assembly in a Bacterial Mangrove Endophyte

Zhongli Xu, Martin Baunach, Ling Ding, Huiyun Peng, Jakob Franke, Christian Hertweck

Research output: Contribution to journalJournal articleCommunication

Abstract

Divergolides are structurally diverse ansamycins produced by a bacterial endophyte (Streptomyces sp.) of the mangrove tree Bruguiera gymnorrhiza. By genomic analyses a gene locus coding for the divergolide pathway was detected. The div gene cluster encodes genes for the biosynthesis of 3-amino-5-hydroxybenzoate and the rare extender units ethylmalonyl-CoA and isobutylmalonyl-CoA, polyketide assembly by a modular type I polyketide synthase (PKS), and enzymes involved in tailoring reactions, such as a Baeyer-Villiger oxygenase. A detailed PKS domain analysis confirmed the stereochemical integrity of the divergolides and provided valuable new insights into the formation of the diverse aromatic chromophores. The bioinformatic analyses and the isolation and full structural elucidation of four new divergolide congeners led to a revised biosynthetic model that illustrates the formation of four different types of ansamycin chromophores from a single polyketide precursor.
Original languageEnglish
JournalChembiochem
Volume15
Issue number9
Pages (from-to)1274-1279
Number of pages6
ISSN1439-4227
DOIs
Publication statusPublished - 2014
Externally publishedYes

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