Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus

Research output: Contribution to journalJournal article – Annual report year: 2017Researchpeer-review

Without internal affiliation

DOI

  • Author: Lee, Sang-Woo

    Korea University

  • Author: Oh, Min-Kuy

    Korea University, Korea, Republic of

View graph of relations

Glucosamine and its derivatives are utilized in the food and biomedical industries. However, current production relies on hydrolysis of natural sources, making it difficult to maintain quality and eliminate allergenic risk. Therefore, microbial production with aid of metabolic engineering is required. We previously demonstrated production of N-acetylglucosamine (GlcNAc) in Saccharomyces cerevisiae by overexpressing an allosteric regulation-free Gfa1p mutant and the haloacid dehalogenase-like phosphatase YqaB. In this study, we further improved GlcNAc production by reducing glycolytic flux. Eukaryotic phosphofructokinase 1 (PFK-1) is allosterically activated by fructose 2,6-bisphosphate (F26BP). Disruption of PFK-2, which synthesizes F26BP, resulted in a slight decrease of GlcNAc production and no significant change of glucose consumption and ethanol production. However, when galactose was used as a sole carbon source to the strain without PFK-2, GlcNAc production was significantly increased and ethanol production was reduced, suggesting that further reduction of glycolytic flux can be used to further improve GlcNAc production. The methodology used in this study can be applied to improve production of carbohydrate derivatives in S. cerevisiae.
Original languageEnglish
JournalBiotechnology and Bioengineering (Print)
Volume113
Issue number11
Pages (from-to)2524-2528
ISSN0006-3592
DOIs
Publication statusPublished - 2016
Externally publishedYes
CitationsWeb of Science® Times Cited: No match on DOI

ID: 133478167