TY - JOUR
T1 - Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus
AU - Kim, Tae-Yeon
AU - Lee, Sang-Woo
AU - Oh, Min-Kyu
PY - 2014
Y1 - 2014
N2 - 2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0 g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroG fbr from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3 g/L of 2-PE was produced from 20 g/L glucose without addition of phenylalanine in the medium.
AB - 2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0 g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroG fbr from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3 g/L of 2-PE was produced from 20 g/L glucose without addition of phenylalanine in the medium.
U2 - 10.1016/j.enzmictec.2014.04.011
DO - 10.1016/j.enzmictec.2014.04.011
M3 - Journal article
SN - 0141-0229
VL - 61-62
SP - 44
EP - 47
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
ER -