Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus

Tae-Yeon Kim, Sang-Woo Lee, Min-Kyu Oh

Research output: Contribution to journalJournal articleResearchpeer-review


2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0 g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroG fbr from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3 g/L of 2-PE was produced from 20 g/L glucose without addition of phenylalanine in the medium.
Original languageEnglish
JournalEnzyme and Microbial Technology
Pages (from-to)44-47
Publication statusPublished - 2014
Externally publishedYes

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