We report on a novel microfluidic system designed to monitor in real-time the concentration of live and dead cells in industrial cell production. Custom-made stepper motor actuated peristaltic pumps and valves, fluidic interconnections, sample-to-waste liquid management and image cytometry-based detection contribute to the high programmability and automation of this platform. Furthermore, this is to the best of our knowledge, the first use of Dean vortices to implement a wide range of dilution factors to highly concentrated cell samples. The combination of a curved channel geometry and high flow rates enables the rapid passive mixing and homogenization of the diluted cell plug.
|Title of host publication||19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015|
|Number of pages||3|
|Publisher||Chemical and Biological Microsystems Society|
|Publication status||Published - 2015|
|Event||MicroTAS 2015: 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Gyeongju, Korea, Republic of|
Duration: 25 Oct 2015 → 29 Oct 2015
Conference number: 19
|Country||Korea, Republic of|
|Period||25/10/2015 → 29/10/2015|
- Microfluidic system
- Cell sample dilution
Rodrigues de Sousa Nunes, P. A., Kjaerulff, S., Dufva, M., & Mogensen, K. B. (2015). Bioreactor process monitoring using an automated microfluidic platform for cell-based assays. In 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 (pp. 1287-1289). Chemical and Biological Microsystems Society.