Abstract
We report on a novel microfluidic system designed to monitor in real-time the concentration of live and dead cells in industrial cell production. Custom-made stepper motor actuated peristaltic pumps and valves, fluidic interconnections, sample-to-waste liquid management and image cytometry-based detection contribute to the high programmability and automation of this platform. Furthermore, this is to the best of our knowledge, the first use of Dean vortices to implement a wide range of dilution factors to highly concentrated cell samples. The combination of a curved channel geometry and high flow rates enables the rapid passive mixing and homogenization of the diluted cell plug.
| Original language | English |
|---|---|
| Title of host publication | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
| Number of pages | 3 |
| Publisher | Chemical and Biological Microsystems Society |
| Publication date | 2015 |
| Pages | 1287-1289 |
| ISBN (Electronic) | 9780979806483 |
| Publication status | Published - 2015 |
| Event | MicroTAS 2015: 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 Conference number: 19 |
Conference
| Conference | MicroTAS 2015 |
|---|---|
| Number | 19 |
| Country | Korea, Republic of |
| City | Gyeongju |
| Period | 25/10/2015 → 29/10/2015 |
Keywords
- Programmable
- Microfluidic system
- Cell sample dilution
- Mixing
- Bioreactor
Cite this
Rodrigues de Sousa Nunes, P. A., Kjaerulff, S., Dufva, M., & Mogensen, K. B. (2015). Bioreactor process monitoring using an automated microfluidic platform for cell-based assays. In 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 (pp. 1287-1289). Chemical and Biological Microsystems Society.