Bioreactor process monitoring using an automated microfluidic platform for cell-based assays

Pedro André Rodrigues de Sousa Nunes, S. Kjaerulff, Martin Dufva, Klaus Bo Mogensen

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    We report on a novel microfluidic system designed to monitor in real-time the concentration of live and dead cells in industrial cell production. Custom-made stepper motor actuated peristaltic pumps and valves, fluidic interconnections, sample-to-waste liquid management and image cytometry-based detection contribute to the high programmability and automation of this platform. Furthermore, this is to the best of our knowledge, the first use of Dean vortices to implement a wide range of dilution factors to highly concentrated cell samples. The combination of a curved channel geometry and high flow rates enables the rapid passive mixing and homogenization of the diluted cell plug.
    Original languageEnglish
    Title of host publication19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
    Number of pages3
    PublisherChemical and Biological Microsystems Society
    Publication date2015
    Pages1287-1289
    ISBN (Electronic)9780979806483
    Publication statusPublished - 2015
    EventMicroTAS 2015: 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Gyeongju, Korea, Republic of
    Duration: 25 Oct 201529 Oct 2015
    Conference number: 19

    Conference

    ConferenceMicroTAS 2015
    Number19
    CountryKorea, Republic of
    CityGyeongju
    Period25/10/201529/10/2015

    Keywords

    • Programmable
    • Microfluidic system
    • Cell sample dilution
    • Mixing
    • Bioreactor

    Cite this

    Rodrigues de Sousa Nunes, P. A., Kjaerulff, S., Dufva, M., & Mogensen, K. B. (2015). Bioreactor process monitoring using an automated microfluidic platform for cell-based assays. In 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 (pp. 1287-1289). Chemical and Biological Microsystems Society.