Biophysical approaches to study actinoporin-lipid interactions

Juan Palacios-Ortega, Esperanza Rivera-de-Torre, José G. Gavilanes, J. Peter Slotte, Álvaro Martínez-del-Pozo, Sara García-Linares*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Abstract

Protein-lipid interactions are crucial events from a biochemical point of view, like the interaction of proteins with the cell plasma membrane, and their study is of great importance. Actinoporins are a very powerful tool to study this kind of interactions, since they are soluble proteins in an aqueous environment, capable of inserting into membranes when they have the adequate composition. In fact, actinoporins have been used to study protein-lipid interactions for many years now. Sometimes it is not possible to use real biological membranes in the experiments, so model membranes need to be used. This article aims to give a thorough description of many of the techniques used to study actinoporin-lipid interactions, using both biological and model membranes: Hemolysis, release of vesicles content, surface plasmon resonance, isothermal titration calorimetry, fluorescence-based measurements, etc. Some of these techniques measure the actinoporins activity and some measure their binding properties. The combination of all the techniques described can offer valuable information about the thermodynamics and the kinetics of the actinoporin-lipid interaction.
Original languageEnglish
Title of host publicationMethods in Enzymology
Number of pages33
PublisherElsevier
DOIs
Publication statusAccepted/In press - 2021
SeriesMethods in Enzymology
ISSN0076-6879

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