Biomass composition of Arthrospira platensis during cultivation on industrial process water and harvesting

Anita Ljubic*, Hamed Safafar, Susan Løvstad Holdt, Charlotte Jacobsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Microalgae have the ability to utilize nutrients from wastewater and use it for biomass production. The effluent from a biogas process was tested as a nutrient source for blue-green microalga Arthrospira platensis cultivation and compared with conventional synthetic medium. Cultivation was carried out in four different concentrations of industrial process water (25, 50, 75, and 100%). The biomass was then harvested by microfiltration, and centrifugation followed by freeze drying. Variations in biomass composition were studied, in order to investigate effects of industrial process water on A. platensis over 30 days of cultivation. Applied harvesting techniques were evaluated for their effect on physiochemical properties of the biomass. Arthrospira platensis was able to grow in all tested wastewater concentrations except 100%, however, increase of wastewater concentration in medium resulted in a decreased growth rate. Partial substitution of synthetic Zarrouk medium with 25% of wastewater showed no adverse effect on chemical composition of the biomass including high protein content (45–58% dry weight) and favorable fatty acid composition (42–45% PUFAs of total fatty acids). Evaluation by optical microscopy showed that microfiltration caused cell rupture at the moderate level while centrifugation had more severe effect on A. platensis. Effect of centrifugal forces and shear stress on A. platensis cells was confirmed by detecting lower lipid content in samples after applying both microfiltration and centrifugation due to cell content leakage.
Original languageEnglish
JournalJournal of Applied Phycology
Volume30
Issue number2
Pages (from-to) 943–954
ISSN0921-8971
DOIs
Publication statusPublished - 2018

Keywords

  • Aquatic Science
  • Plant Science
  • Biomass composition
  • Cell rupture
  • Centrifugation
  • Cross-flow microfiltration
  • Spirulina
  • Wastewater

Cite this

@article{13bfc8fbb78341369f580845175f58ba,
title = "Biomass composition of Arthrospira platensis during cultivation on industrial process water and harvesting",
abstract = "Microalgae have the ability to utilize nutrients from wastewater and use it for biomass production. The effluent from a biogas process was tested as a nutrient source for blue-green microalga Arthrospira platensis cultivation and compared with conventional synthetic medium. Cultivation was carried out in four different concentrations of industrial process water (25, 50, 75, and 100{\%}). The biomass was then harvested by microfiltration, and centrifugation followed by freeze drying. Variations in biomass composition were studied, in order to investigate effects of industrial process water on A. platensis over 30 days of cultivation. Applied harvesting techniques were evaluated for their effect on physiochemical properties of the biomass. Arthrospira platensis was able to grow in all tested wastewater concentrations except 100{\%}, however, increase of wastewater concentration in medium resulted in a decreased growth rate. Partial substitution of synthetic Zarrouk medium with 25{\%} of wastewater showed no adverse effect on chemical composition of the biomass including high protein content (45{\^a}€“58{\%} dry weight) and favorable fatty acid composition (42{\^a}€“45{\%} PUFAs of total fatty acids). Evaluation by optical microscopy showed that microfiltration caused cell rupture at the moderate level while centrifugation had more severe effect on A. platensis. Effect of centrifugal forces and shear stress on A. platensis cells was confirmed by detecting lower lipid content in samples after applying both microfiltration and centrifugation due to cell content leakage.",
keywords = "Aquatic Science, Plant Science, Biomass composition, Cell rupture, Centrifugation, Cross-flow microfiltration, Spirulina, Wastewater",
author = "Anita Ljubic and Hamed Safafar and Holdt, {Susan L{\o}vstad} and Charlotte Jacobsen",
year = "2018",
doi = "10.1007/s10811-017-1332-y",
language = "English",
volume = "30",
pages = "943–954",
journal = "Journal of Applied Phycology",
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Biomass composition of Arthrospira platensis during cultivation on industrial process water and harvesting. / Ljubic, Anita; Safafar, Hamed; Holdt, Susan Løvstad; Jacobsen, Charlotte.

In: Journal of Applied Phycology, Vol. 30, No. 2, 2018, p. 943–954.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Biomass composition of Arthrospira platensis during cultivation on industrial process water and harvesting

AU - Ljubic, Anita

AU - Safafar, Hamed

AU - Holdt, Susan Løvstad

AU - Jacobsen, Charlotte

PY - 2018

Y1 - 2018

N2 - Microalgae have the ability to utilize nutrients from wastewater and use it for biomass production. The effluent from a biogas process was tested as a nutrient source for blue-green microalga Arthrospira platensis cultivation and compared with conventional synthetic medium. Cultivation was carried out in four different concentrations of industrial process water (25, 50, 75, and 100%). The biomass was then harvested by microfiltration, and centrifugation followed by freeze drying. Variations in biomass composition were studied, in order to investigate effects of industrial process water on A. platensis over 30 days of cultivation. Applied harvesting techniques were evaluated for their effect on physiochemical properties of the biomass. Arthrospira platensis was able to grow in all tested wastewater concentrations except 100%, however, increase of wastewater concentration in medium resulted in a decreased growth rate. Partial substitution of synthetic Zarrouk medium with 25% of wastewater showed no adverse effect on chemical composition of the biomass including high protein content (45–58% dry weight) and favorable fatty acid composition (42–45% PUFAs of total fatty acids). Evaluation by optical microscopy showed that microfiltration caused cell rupture at the moderate level while centrifugation had more severe effect on A. platensis. Effect of centrifugal forces and shear stress on A. platensis cells was confirmed by detecting lower lipid content in samples after applying both microfiltration and centrifugation due to cell content leakage.

AB - Microalgae have the ability to utilize nutrients from wastewater and use it for biomass production. The effluent from a biogas process was tested as a nutrient source for blue-green microalga Arthrospira platensis cultivation and compared with conventional synthetic medium. Cultivation was carried out in four different concentrations of industrial process water (25, 50, 75, and 100%). The biomass was then harvested by microfiltration, and centrifugation followed by freeze drying. Variations in biomass composition were studied, in order to investigate effects of industrial process water on A. platensis over 30 days of cultivation. Applied harvesting techniques were evaluated for their effect on physiochemical properties of the biomass. Arthrospira platensis was able to grow in all tested wastewater concentrations except 100%, however, increase of wastewater concentration in medium resulted in a decreased growth rate. Partial substitution of synthetic Zarrouk medium with 25% of wastewater showed no adverse effect on chemical composition of the biomass including high protein content (45–58% dry weight) and favorable fatty acid composition (42–45% PUFAs of total fatty acids). Evaluation by optical microscopy showed that microfiltration caused cell rupture at the moderate level while centrifugation had more severe effect on A. platensis. Effect of centrifugal forces and shear stress on A. platensis cells was confirmed by detecting lower lipid content in samples after applying both microfiltration and centrifugation due to cell content leakage.

KW - Aquatic Science

KW - Plant Science

KW - Biomass composition

KW - Cell rupture

KW - Centrifugation

KW - Cross-flow microfiltration

KW - Spirulina

KW - Wastewater

U2 - 10.1007/s10811-017-1332-y

DO - 10.1007/s10811-017-1332-y

M3 - Journal article

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SN - 0921-8971

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