Biomarkers for exposure to ambient air pollution - Comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress

Herman Autrup, Bahram Daneshvar, Lars Ove Dragsted, Mikael Gamborg, Åse Marie Hansen, Steffen Loft, Henrik Okkels, Flemming Nielsen, Per Sabro Nielsen, Edith Raffn, Håkan Wallin, Lisbeth Ehlert Knudsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Human exposure to genotoxic compounds present in ambient air has been studied using selected biomarkers in nonsmoking Danish bus drivers and postal workers. A large interindividual variation in biomarker levels was observed. Significantly higher levels of bulky carcinogen-DNA adducts (75.42 adducts/10(8) nucleotides) and of 2-amino-apidic semialdehyde (AAS) in plasma proteins (56.7 pmol/mg protein) were observed in bus drivers working in the central part of Copenhagen, Denmark. In contrast, significantly higher levels of AAS in hemoglobin (55.8 pmol/mg protein), malondialdehyde in plasma (0.96 nmol/ml plasma), and polycyclic aromatic hydrocarbon (PAH)-albumin adduct (3.38 fmol/mu g albumin) were observed in the suburban group. The biomarker levels in postal workers were similar to the levels in suburban bus drivers. In the combined group of bus drivers and postal workers, negative correlations were observed between bulky carcinogen-DNA adduct and PAM-albumin levels (p = 0.005), and between DNA adduct and gamma-glutamyl semialdehyde (GGS) in hemoglobin (p = 0.11). Highly significant correlations were found between PAM-albumin adducts and AAS in plasma (r = 0.001) and GGS in hemoglobin (p = 0.001). Significant correlations were also observed between urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine and AAS in plasma (p = 0.001) and PAM-albumin adducts (p = 0.002). The influence of the glutatione S-transferase (GST) M1 deletion on the correlation between the biomarkers was studied in the combined group. A significant negative correlation was only observed between bulky carcinogen-DNA adducts and PAM-albumin adducts (p = 0.02) and between DNA adduct and urinary mutagenic activity (p = 0.02) in the GSTM1 null group, bur not in the workers who were homozygotes or heterozygotes for GSTM1. Our results indicate that some of the selected biomarkers can be used to distinguish between high and low exposure to environmental genotoxins.
Original languageEnglish
JournalEnvironmental Health Perspectives
Volume107
Issue number3
Pages (from-to)233-238
ISSN0091-6765
Publication statusPublished - 1999

Keywords

  • genotoxic exposure
  • adducts
  • biomarkers
  • oxidative stress
  • human
  • air pollution

Cite this

Autrup, H., Daneshvar, B., Dragsted, L. O., Gamborg, M., Hansen, Å. M., Loft, S., Okkels, H., Nielsen, F., Nielsen, P. S., Raffn, E., Wallin, H., & Knudsen, L. E. (1999). Biomarkers for exposure to ambient air pollution - Comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress. Environmental Health Perspectives, 107(3), 233-238.