Biological Potential of Chitinolytic Marine Bacteria

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Chitinolytic microorganisms secrete a range of chitin modifying enzymes, which can be exploited for production of chitin derived products or as fungal or pest control agents. Here, we explored the potential of 11 marine bacteria (Pseudoalteromonadaceae, Vibrionaceae) for chitin degradation using in silico and phenotypic assays. Of 10 chitinolytic strains, three strains, Photobacterium galatheae S2753, Pseudoalteromonas piscicida S2040 and S2724, produced large clearing zones on chitin plates. All strains were antifungal, but against different fungal targets. One strain, Pseudoalteromonas piscicida S2040, had a pronounced antifungal activity against all seven fungal strains. There was no correlation between the number of chitin modifying enzymes as found by genome mining and the chitin degrading activity as measured by size of clearing zones on chitin agar. Based on in silico and in vitro analyses, we cloned and expressed two ChiA-like chitinases from the two most potent candidates to exemplify the industrial potential.
Original languageEnglish
JournalMarine Drugs
Volume14
Issue number12
Pages (from-to)230
Number of pages1
ISSN1660-3397
DOIs
Publication statusPublished - 2016

Keywords

  • Pseudoalteromonadaceae
  • Vibrionaceae
  • antifungal
  • chitin
  • chitinases
  • marine bacteria
  • Biology (General)
  • QH301-705.5
  • Science
  • Q
  • Therapeutics. Pharmacology
  • RM1-950
  • Medicine
  • R

Cite this

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title = "Biological Potential of Chitinolytic Marine Bacteria",
abstract = "Chitinolytic microorganisms secrete a range of chitin modifying enzymes, which can be exploited for production of chitin derived products or as fungal or pest control agents. Here, we explored the potential of 11 marine bacteria (Pseudoalteromonadaceae, Vibrionaceae) for chitin degradation using in silico and phenotypic assays. Of 10 chitinolytic strains, three strains, Photobacterium galatheae S2753, Pseudoalteromonas piscicida S2040 and S2724, produced large clearing zones on chitin plates. All strains were antifungal, but against different fungal targets. One strain, Pseudoalteromonas piscicida S2040, had a pronounced antifungal activity against all seven fungal strains. There was no correlation between the number of chitin modifying enzymes as found by genome mining and the chitin degrading activity as measured by size of clearing zones on chitin agar. Based on in silico and in vitro analyses, we cloned and expressed two ChiA-like chitinases from the two most potent candidates to exemplify the industrial potential.",
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author = "Paulsen, {Sara Sk{\o}tt} and Birgitte Andersen and Lone Gram and Henrique Machado",
year = "2016",
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language = "English",
volume = "14",
pages = "230",
journal = "Marine Drugs",
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Biological Potential of Chitinolytic Marine Bacteria. / Paulsen, Sara Skøtt; Andersen, Birgitte; Gram, Lone; Machado, Henrique.

In: Marine Drugs, Vol. 14, No. 12, 2016, p. 230.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Biological Potential of Chitinolytic Marine Bacteria

AU - Paulsen, Sara Skøtt

AU - Andersen, Birgitte

AU - Gram, Lone

AU - Machado, Henrique

PY - 2016

Y1 - 2016

N2 - Chitinolytic microorganisms secrete a range of chitin modifying enzymes, which can be exploited for production of chitin derived products or as fungal or pest control agents. Here, we explored the potential of 11 marine bacteria (Pseudoalteromonadaceae, Vibrionaceae) for chitin degradation using in silico and phenotypic assays. Of 10 chitinolytic strains, three strains, Photobacterium galatheae S2753, Pseudoalteromonas piscicida S2040 and S2724, produced large clearing zones on chitin plates. All strains were antifungal, but against different fungal targets. One strain, Pseudoalteromonas piscicida S2040, had a pronounced antifungal activity against all seven fungal strains. There was no correlation between the number of chitin modifying enzymes as found by genome mining and the chitin degrading activity as measured by size of clearing zones on chitin agar. Based on in silico and in vitro analyses, we cloned and expressed two ChiA-like chitinases from the two most potent candidates to exemplify the industrial potential.

AB - Chitinolytic microorganisms secrete a range of chitin modifying enzymes, which can be exploited for production of chitin derived products or as fungal or pest control agents. Here, we explored the potential of 11 marine bacteria (Pseudoalteromonadaceae, Vibrionaceae) for chitin degradation using in silico and phenotypic assays. Of 10 chitinolytic strains, three strains, Photobacterium galatheae S2753, Pseudoalteromonas piscicida S2040 and S2724, produced large clearing zones on chitin plates. All strains were antifungal, but against different fungal targets. One strain, Pseudoalteromonas piscicida S2040, had a pronounced antifungal activity against all seven fungal strains. There was no correlation between the number of chitin modifying enzymes as found by genome mining and the chitin degrading activity as measured by size of clearing zones on chitin agar. Based on in silico and in vitro analyses, we cloned and expressed two ChiA-like chitinases from the two most potent candidates to exemplify the industrial potential.

KW - Pseudoalteromonadaceae

KW - Vibrionaceae

KW - antifungal

KW - chitin

KW - chitinases

KW - marine bacteria

KW - Biology (General)

KW - QH301-705.5

KW - Science

KW - Q

KW - Therapeutics. Pharmacology

KW - RM1-950

KW - Medicine

KW - R

U2 - 10.3390/md14120230

DO - 10.3390/md14120230

M3 - Journal article

VL - 14

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JO - Marine Drugs

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SN - 1660-3397

IS - 12

ER -