Biological caproate production by Clostridium kluyveri from ethanol and acetate as carbon sources

Yanan Yin, Yifeng Zhang, Dimitar Borisov Karakashev, Jianlong Wang, Irini Angelidaki

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Caproate is a valuable industrial product and chemical precursor. In this study, batch tests were conducted to investigate the fermentative caproate production through chain elongation from acetate and ethanol. The effect of acetate/ethanol ratio and initial ethanol concentration on caproate production was examined. When substrate concentration was controlled at 100 mM total carbon, hydrogen was used as an additional electron donor. The highest caproate concentration of 3.11 g/L was obtained at an ethanol/acetate ratio of 7:3. No additional electron donor was needed upon an ethanol/acetate ratio ≥7:3. Caproate production increased with the increase of carbon source until ethanol concentration over 700 mM, which inhibited the fermentation process. The highest caproate concentration of 8.42 g/L was achieved from high ethanol strength wastewater with an ethanol/acetate ratio of 10:1 (550 mM total carbon). Results obtained in this study can pave the way towards efficient chain elongation from ethanol-rich wastewater.
Original languageEnglish
JournalBioresource Technology
Pages (from-to)638-644
Publication statusPublished - 2017


  • Chain elongation
  • n-caproate
  • Clostridium kluyveri
  • Fermentation
  • Ethanol/acetate ratio

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