Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors

Research output: Contribution to journalJournal article – Annual report year: 2017Researchpeer-review

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  • Author: Choi, Won Il

    Korea Institute of Ceramic Engineering And Technology, Korea, Republic of

  • Author: Sahu, Abhishek

    Gwangju Institute of Science and Technology, Korea, Republic of

  • Author: Vilos, Cristian

    Universidad Andres Bello, Chile

  • Author: Kamaly, Nazila

    Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Jo, Seong-Min

    Max Planck Institute for Polymer Research, Germany

  • Author: Lee, Jin Hyung

    Korea Institute of Ceramic Engineering And Technology, Korea, Republic of

  • Author: Tae, Giyoong

    Gwangju Institute of Science and Technology, Korea, Republic of

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Growth factors have great therapeutic potential for various disease therapy and tissue engineering applications. However, their clinical efficacy is hampered by low bioavailability, rapid degradation in vivo and non-specific biodistribution. Nanoparticle based delivery systems are being evaluated to overcome these limitations. Herein, we have developed a thermosensitive heparin nanosponge (Hep-NS) by a one step photopolymerization reaction between diacrylated pluronic and thiolated heparin molecules. The amount of heparin in Hep-NS was precisely controlled by varying the heparin amount in the reaction feed. Hep-NS with varying amounts of heparin showed similar size and shape properties, though surface charge decreased with an increase in the amount of heparin conjugation. The anticoagulant activity of the Hep-NS decreased by 65% compared to free heparin, however the Hep-NS retained their growth factor binding ability. Four different growth factors, bFGF, VEGF, BMP-2, and HGF were successfully encapsulated into Hep-NS. In vitro studies showed sustained release of all the growth factors for almost 60 days and the rate of release was directly dependent on the amount of heparin in Hep-NS. The released growth factors retained their bioactivity as assessed by a cell proliferation assay. This heparin nanosponge is therefore a promising nanocarrier for the loading and controlled release of growth factors.
Original languageEnglish
Article number14351
JournalScientific Reports
Volume7
Issue number1
ISSN2045-2322
DOIs
Publication statusPublished - 2017
CitationsWeb of Science® Times Cited: No match on DOI

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