Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors

Won Il Choi, Abhishek Sahu, Cristian Vilos, Nazila Kamaly, Seong-Min Jo, Jin Hyung Lee, Giyoong Tae

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    Growth factors have great therapeutic potential for various disease therapy and tissue engineering applications. However, their clinical efficacy is hampered by low bioavailability, rapid degradation in vivo and non-specific biodistribution. Nanoparticle based delivery systems are being evaluated to overcome these limitations. Herein, we have developed a thermosensitive heparin nanosponge (Hep-NS) by a one step photopolymerization reaction between diacrylated pluronic and thiolated heparin molecules. The amount of heparin in Hep-NS was precisely controlled by varying the heparin amount in the reaction feed. Hep-NS with varying amounts of heparin showed similar size and shape properties, though surface charge decreased with an increase in the amount of heparin conjugation. The anticoagulant activity of the Hep-NS decreased by 65% compared to free heparin, however the Hep-NS retained their growth factor binding ability. Four different growth factors, bFGF, VEGF, BMP-2, and HGF were successfully encapsulated into Hep-NS. In vitro studies showed sustained release of all the growth factors for almost 60 days and the rate of release was directly dependent on the amount of heparin in Hep-NS. The released growth factors retained their bioactivity as assessed by a cell proliferation assay. This heparin nanosponge is therefore a promising nanocarrier for the loading and controlled release of growth factors.
    Original languageEnglish
    Article number14351
    JournalScientific Reports
    Volume7
    Issue number1
    ISSN2045-2322
    DOIs
    Publication statusPublished - 2017

    Cite this

    Choi, Won Il ; Sahu, Abhishek ; Vilos, Cristian ; Kamaly, Nazila ; Jo, Seong-Min ; Lee, Jin Hyung ; Tae, Giyoong. / Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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    title = "Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors",
    abstract = "Growth factors have great therapeutic potential for various disease therapy and tissue engineering applications. However, their clinical efficacy is hampered by low bioavailability, rapid degradation in vivo and non-specific biodistribution. Nanoparticle based delivery systems are being evaluated to overcome these limitations. Herein, we have developed a thermosensitive heparin nanosponge (Hep-NS) by a one step photopolymerization reaction between diacrylated pluronic and thiolated heparin molecules. The amount of heparin in Hep-NS was precisely controlled by varying the heparin amount in the reaction feed. Hep-NS with varying amounts of heparin showed similar size and shape properties, though surface charge decreased with an increase in the amount of heparin conjugation. The anticoagulant activity of the Hep-NS decreased by 65{\%} compared to free heparin, however the Hep-NS retained their growth factor binding ability. Four different growth factors, bFGF, VEGF, BMP-2, and HGF were successfully encapsulated into Hep-NS. In vitro studies showed sustained release of all the growth factors for almost 60 days and the rate of release was directly dependent on the amount of heparin in Hep-NS. The released growth factors retained their bioactivity as assessed by a cell proliferation assay. This heparin nanosponge is therefore a promising nanocarrier for the loading and controlled release of growth factors.",
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    Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors. / Choi, Won Il; Sahu, Abhishek; Vilos, Cristian; Kamaly, Nazila; Jo, Seong-Min; Lee, Jin Hyung; Tae, Giyoong.

    In: Scientific Reports, Vol. 7, No. 1, 14351, 2017.

    Research output: Contribution to journalJournal articleResearchpeer-review

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    T1 - Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors

    AU - Choi, Won Il

    AU - Sahu, Abhishek

    AU - Vilos, Cristian

    AU - Kamaly, Nazila

    AU - Jo, Seong-Min

    AU - Lee, Jin Hyung

    AU - Tae, Giyoong

    PY - 2017

    Y1 - 2017

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    AB - Growth factors have great therapeutic potential for various disease therapy and tissue engineering applications. However, their clinical efficacy is hampered by low bioavailability, rapid degradation in vivo and non-specific biodistribution. Nanoparticle based delivery systems are being evaluated to overcome these limitations. Herein, we have developed a thermosensitive heparin nanosponge (Hep-NS) by a one step photopolymerization reaction between diacrylated pluronic and thiolated heparin molecules. The amount of heparin in Hep-NS was precisely controlled by varying the heparin amount in the reaction feed. Hep-NS with varying amounts of heparin showed similar size and shape properties, though surface charge decreased with an increase in the amount of heparin conjugation. The anticoagulant activity of the Hep-NS decreased by 65% compared to free heparin, however the Hep-NS retained their growth factor binding ability. Four different growth factors, bFGF, VEGF, BMP-2, and HGF were successfully encapsulated into Hep-NS. In vitro studies showed sustained release of all the growth factors for almost 60 days and the rate of release was directly dependent on the amount of heparin in Hep-NS. The released growth factors retained their bioactivity as assessed by a cell proliferation assay. This heparin nanosponge is therefore a promising nanocarrier for the loading and controlled release of growth factors.

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