Bioinformatically predicted emulsifying peptides and potato protein hydrolysate improves the oxidative stability of microencapsulated fish oil

Mads Bjørlie*, Betül Yesiltas, Pedro J. García-Moreno, F. Javier Espejo-Carpio, Nor E. Rahmani-Manglano, Emilia M. Guadix, Ali Jafarpour, Egon B. Hansen, Paolo Marcatili, Michael T. Overgaard, Simon Gregersen Echers, Charlotte Jacobsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The aim of this study was to investigate the potential of potato proteins and peptides as emulsifiers in the microencapsulation of fish oil by spray-drying. Microcapsules were produced using a potato protein extract, and fractions enriched in patatin and protease inhibitors. Furthermore, bioinformatically predicted emulsifier peptides from abundant potato proteins and a hydrolysate, obtained through targeted proteolysis of the extract, were investigated. During 28 days of storage at 25 °C, peptides and the hydrolysate exhibited better emulsifying properties and higher encapsulation efficiencies compared to native proteins and sodium caseinate. Significant differences (p < 0.05) were observed in the peroxide value (PV) and secondary volatile oxidation products between the microcapsules produced with peptides and native proteins. Microcapsules produced with peptides and hydrolysate showed the highest oxidative stability, not exceeding a PV of 10 meq/kg oil, and with concentrations of volatiles below the odor threshold in oil for five of the six studied compounds. These results show the emulsifying potential of potato peptides and hydrolysate for use in microencapsulation of hydrophobic bioactive ingredients such as fish oil.
Original languageEnglish
Article number100441
JournalFood Chemistry Advances
Volume3
Number of pages11
ISSN2772-753x
DOIs
Publication statusPublished - 2023

Keywords

  • Fish oil
  • Lipid oxidation
  • Microencapsulation
  • Emulsifiers
  • Bioinformatics
  • Potato proteins
  • Hydrolysates
  • Spray-drying

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