Bioimpedance monitoring of 3D cell culturing-Complementary electrode configurations for enhanced spatial sensitivity

Research output: Contribution to journalJournal article – Annual report year: 2015Researchpeer-review

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Bioimpedance monitoring of 3D cell culturing-Complementary electrode configurations for enhanced spatial sensitivity. / Canali, Chiara; Heiskanen, Arto; Muhammad, Haseena Bashir; Høyum, Per; Pettersen, Fred-Johan; Hemmingsen, Mette; Wolff, Anders; Dufva, Martin; Martinsen, Orjan Grøttem; Emnéus, Jenny.

In: Biosensors and Bioelectronics, Vol. 63, 2015, p. 72-79.

Research output: Contribution to journalJournal article – Annual report year: 2015Researchpeer-review

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@article{d82a851f2ab44e8391bbfe37c8f8ac11,
title = "Bioimpedance monitoring of 3D cell culturing-Complementary electrode configurations for enhanced spatial sensitivity",
abstract = "A bioimpedance platform is presented as a promising tool for non-invasive real-time monitoring of the entire process of three-dimensional (3D) cell culturing in a hydrogel scaffold. In this study, the dynamics involved in the whole process of 3D cell culturing, starting from polymerisation of a bare 3D gelatin scaffold, to human mesenchymal stem cell (MSC) encapsulation and proliferation, was monitored over time. The platform consists of a large rectangular culture chamber with four embedded vertical gold plate electrodes that were exploited in two- and three terminal (2T and 3T) measurement configurations. By switching between the different combinations of electrode couples, it was possible to generate a multiplexing-like approach, which allowed for collecting spatially distributed information within the 3D space. Computational finite element (FE) analysis and electrochemical impedance spectroscopic (EIS) characterisation were used to determine the configurations' sensitivity field localisation. The 2T setup gives insight into the interfacial phenomena at both electrode surfaces and covers the central part of the 3D cell culture volume, while the four 3T modes provide focus on the dynamics at the corners of the 3D culture chamber. By combining a number of electrode configurations, complementary spatially distributed information on a large 3D cell culture can be obtained with maximised sensitivity in the entire 3D space. The experimental results show that cell proliferation can be monitored within the tested biomimetic environment, paving the way to further developments in bioimpedance tracking of 3D cell cultures and tissue engineering.",
keywords = "Bioimpedance, 3D cell culture, Gelatin scaffold, Multiplexed measurements, Three-terminal impedance, Sensitivity field distribution",
author = "Chiara Canali and Arto Heiskanen and Muhammad, {Haseena Bashir} and Per H{\o}yum and Fred-Johan Pettersen and Mette Hemmingsen and Anders Wolff and Martin Dufva and Martinsen, {Orjan Gr{\o}ttem} and Jenny Emn{\'e}us",
year = "2015",
doi = "10.1016/j.bios.2014.07.020",
language = "English",
volume = "63",
pages = "72--79",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier B.V.",

}

RIS

TY - JOUR

T1 - Bioimpedance monitoring of 3D cell culturing-Complementary electrode configurations for enhanced spatial sensitivity

AU - Canali, Chiara

AU - Heiskanen, Arto

AU - Muhammad, Haseena Bashir

AU - Høyum, Per

AU - Pettersen, Fred-Johan

AU - Hemmingsen, Mette

AU - Wolff, Anders

AU - Dufva, Martin

AU - Martinsen, Orjan Grøttem

AU - Emnéus, Jenny

PY - 2015

Y1 - 2015

N2 - A bioimpedance platform is presented as a promising tool for non-invasive real-time monitoring of the entire process of three-dimensional (3D) cell culturing in a hydrogel scaffold. In this study, the dynamics involved in the whole process of 3D cell culturing, starting from polymerisation of a bare 3D gelatin scaffold, to human mesenchymal stem cell (MSC) encapsulation and proliferation, was monitored over time. The platform consists of a large rectangular culture chamber with four embedded vertical gold plate electrodes that were exploited in two- and three terminal (2T and 3T) measurement configurations. By switching between the different combinations of electrode couples, it was possible to generate a multiplexing-like approach, which allowed for collecting spatially distributed information within the 3D space. Computational finite element (FE) analysis and electrochemical impedance spectroscopic (EIS) characterisation were used to determine the configurations' sensitivity field localisation. The 2T setup gives insight into the interfacial phenomena at both electrode surfaces and covers the central part of the 3D cell culture volume, while the four 3T modes provide focus on the dynamics at the corners of the 3D culture chamber. By combining a number of electrode configurations, complementary spatially distributed information on a large 3D cell culture can be obtained with maximised sensitivity in the entire 3D space. The experimental results show that cell proliferation can be monitored within the tested biomimetic environment, paving the way to further developments in bioimpedance tracking of 3D cell cultures and tissue engineering.

AB - A bioimpedance platform is presented as a promising tool for non-invasive real-time monitoring of the entire process of three-dimensional (3D) cell culturing in a hydrogel scaffold. In this study, the dynamics involved in the whole process of 3D cell culturing, starting from polymerisation of a bare 3D gelatin scaffold, to human mesenchymal stem cell (MSC) encapsulation and proliferation, was monitored over time. The platform consists of a large rectangular culture chamber with four embedded vertical gold plate electrodes that were exploited in two- and three terminal (2T and 3T) measurement configurations. By switching between the different combinations of electrode couples, it was possible to generate a multiplexing-like approach, which allowed for collecting spatially distributed information within the 3D space. Computational finite element (FE) analysis and electrochemical impedance spectroscopic (EIS) characterisation were used to determine the configurations' sensitivity field localisation. The 2T setup gives insight into the interfacial phenomena at both electrode surfaces and covers the central part of the 3D cell culture volume, while the four 3T modes provide focus on the dynamics at the corners of the 3D culture chamber. By combining a number of electrode configurations, complementary spatially distributed information on a large 3D cell culture can be obtained with maximised sensitivity in the entire 3D space. The experimental results show that cell proliferation can be monitored within the tested biomimetic environment, paving the way to further developments in bioimpedance tracking of 3D cell cultures and tissue engineering.

KW - Bioimpedance

KW - 3D cell culture

KW - Gelatin scaffold

KW - Multiplexed measurements

KW - Three-terminal impedance

KW - Sensitivity field distribution

U2 - 10.1016/j.bios.2014.07.020

DO - 10.1016/j.bios.2014.07.020

M3 - Journal article

VL - 63

SP - 72

EP - 79

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

ER -