Biogeography of the fish pathogen Aeromonas salmonicida inferred by vapA genotyping

Snorre Gulla*, Sion Bayliss, Bryndís Björnsdóttir, Inger Dalsgaard, Olga Haenen, Eva Jansson, Una McCarthy, Felix Scholz, Maaike Vercauteren, David W. Verner-Jeffreys, Timothy Welch, Tom Wiklund, Duncan J. Colquhoun

*Corresponding author for this work

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Abstract

A recently described typing system based on sequence variation in the virulence array protein (vapA) gene, encoding the A-layer surface protein array, allows unambiguous subtyping of Aeromonas salmonicida. In the present study we compile A-layer typing results from a total of 675 A. salmonicida isolates, recovered over a 59-year period from 50 different fish species in 26 countries. Nine novel A-layer types (15–23) are identified, several of which display a strong predilection towards certain fish hosts, including Cyprinidae- and Pleuronectidae species. Moreover, we find indications that anthropogenic transport of live fish may have aided the near global dissemination of two cyprinid-associated A-layer types. Comparison of whole genome phylogeny and A-layer typing for a subset of strains further resulted in compatible tree topologies, indicating the utility of vapA as a phylogenetic as well as an epizootiological marker in A. salmonicida. A Microreact project (microreact.org/project/r1pcOAx9m) has been created, allowing public access to the vapA analyses and relevant metadata. In sum, the results generated provide valuable insights into the global population structure of A. salmonicida, particularly in relation to its piscine host spectrum and the geographic distribution of these hosts.
Original languageEnglish
Article numberfnz074
JournalF E M S Microbiology Letters
Volume366
Issue number7
Number of pages12
ISSN0378-1097
DOIs
Publication statusPublished - 2019

Keywords

  • Aquaculture
  • Bacterial fish pathogenp
  • Aeromonas salmonicida
  • VapA
  • A-layer
  • Genotyping
  • Host specificity

Cite this

Gulla, S., Bayliss, S., Björnsdóttir, B., Dalsgaard, I., Haenen, O., Jansson, E., ... Colquhoun, D. J. (2019). Biogeography of the fish pathogen Aeromonas salmonicida inferred by vapA genotyping. F E M S Microbiology Letters, 366(7), [fnz074]. https://doi.org/10.1093/femsle/fnz074
Gulla, Snorre ; Bayliss, Sion ; Björnsdóttir, Bryndís ; Dalsgaard, Inger ; Haenen, Olga ; Jansson, Eva ; McCarthy, Una ; Scholz, Felix ; Vercauteren, Maaike ; Verner-Jeffreys, David W. ; Welch, Timothy ; Wiklund, Tom ; Colquhoun, Duncan J. / Biogeography of the fish pathogen Aeromonas salmonicida inferred by vapA genotyping. In: F E M S Microbiology Letters. 2019 ; Vol. 366, No. 7.
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title = "Biogeography of the fish pathogen Aeromonas salmonicida inferred by vapA genotyping",
abstract = "A recently described typing system based on sequence variation in the virulence array protein (vapA) gene, encoding the A-layer surface protein array, allows unambiguous subtyping of Aeromonas salmonicida. In the present study we compile A-layer typing results from a total of 675 A. salmonicida isolates, recovered over a 59-year period from 50 different fish species in 26 countries. Nine novel A-layer types (15–23) are identified, several of which display a strong predilection towards certain fish hosts, including Cyprinidae- and Pleuronectidae species. Moreover, we find indications that anthropogenic transport of live fish may have aided the near global dissemination of two cyprinid-associated A-layer types. Comparison of whole genome phylogeny and A-layer typing for a subset of strains further resulted in compatible tree topologies, indicating the utility of vapA as a phylogenetic as well as an epizootiological marker in A. salmonicida. A Microreact project (microreact.org/project/r1pcOAx9m) has been created, allowing public access to the vapA analyses and relevant metadata. In sum, the results generated provide valuable insights into the global population structure of A. salmonicida, particularly in relation to its piscine host spectrum and the geographic distribution of these hosts.",
keywords = "Aquaculture, Bacterial fish pathogenp, Aeromonas salmonicida, VapA, A-layer, Genotyping, Host specificity",
author = "Snorre Gulla and Sion Bayliss and Brynd{\'i}s Bj{\"o}rnsd{\'o}ttir and Inger Dalsgaard and Olga Haenen and Eva Jansson and Una McCarthy and Felix Scholz and Maaike Vercauteren and Verner-Jeffreys, {David W.} and Timothy Welch and Tom Wiklund and Colquhoun, {Duncan J.}",
year = "2019",
doi = "10.1093/femsle/fnz074",
language = "English",
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Gulla, S, Bayliss, S, Björnsdóttir, B, Dalsgaard, I, Haenen, O, Jansson, E, McCarthy, U, Scholz, F, Vercauteren, M, Verner-Jeffreys, DW, Welch, T, Wiklund, T & Colquhoun, DJ 2019, 'Biogeography of the fish pathogen Aeromonas salmonicida inferred by vapA genotyping', F E M S Microbiology Letters, vol. 366, no. 7, fnz074. https://doi.org/10.1093/femsle/fnz074

Biogeography of the fish pathogen Aeromonas salmonicida inferred by vapA genotyping. / Gulla, Snorre; Bayliss, Sion; Björnsdóttir, Bryndís ; Dalsgaard, Inger; Haenen, Olga; Jansson, Eva; McCarthy, Una; Scholz, Felix; Vercauteren, Maaike; Verner-Jeffreys, David W.; Welch, Timothy; Wiklund, Tom; Colquhoun, Duncan J.

In: F E M S Microbiology Letters, Vol. 366, No. 7, fnz074, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Biogeography of the fish pathogen Aeromonas salmonicida inferred by vapA genotyping

AU - Gulla, Snorre

AU - Bayliss, Sion

AU - Björnsdóttir, Bryndís

AU - Dalsgaard, Inger

AU - Haenen, Olga

AU - Jansson, Eva

AU - McCarthy, Una

AU - Scholz, Felix

AU - Vercauteren, Maaike

AU - Verner-Jeffreys, David W.

AU - Welch, Timothy

AU - Wiklund, Tom

AU - Colquhoun, Duncan J.

PY - 2019

Y1 - 2019

N2 - A recently described typing system based on sequence variation in the virulence array protein (vapA) gene, encoding the A-layer surface protein array, allows unambiguous subtyping of Aeromonas salmonicida. In the present study we compile A-layer typing results from a total of 675 A. salmonicida isolates, recovered over a 59-year period from 50 different fish species in 26 countries. Nine novel A-layer types (15–23) are identified, several of which display a strong predilection towards certain fish hosts, including Cyprinidae- and Pleuronectidae species. Moreover, we find indications that anthropogenic transport of live fish may have aided the near global dissemination of two cyprinid-associated A-layer types. Comparison of whole genome phylogeny and A-layer typing for a subset of strains further resulted in compatible tree topologies, indicating the utility of vapA as a phylogenetic as well as an epizootiological marker in A. salmonicida. A Microreact project (microreact.org/project/r1pcOAx9m) has been created, allowing public access to the vapA analyses and relevant metadata. In sum, the results generated provide valuable insights into the global population structure of A. salmonicida, particularly in relation to its piscine host spectrum and the geographic distribution of these hosts.

AB - A recently described typing system based on sequence variation in the virulence array protein (vapA) gene, encoding the A-layer surface protein array, allows unambiguous subtyping of Aeromonas salmonicida. In the present study we compile A-layer typing results from a total of 675 A. salmonicida isolates, recovered over a 59-year period from 50 different fish species in 26 countries. Nine novel A-layer types (15–23) are identified, several of which display a strong predilection towards certain fish hosts, including Cyprinidae- and Pleuronectidae species. Moreover, we find indications that anthropogenic transport of live fish may have aided the near global dissemination of two cyprinid-associated A-layer types. Comparison of whole genome phylogeny and A-layer typing for a subset of strains further resulted in compatible tree topologies, indicating the utility of vapA as a phylogenetic as well as an epizootiological marker in A. salmonicida. A Microreact project (microreact.org/project/r1pcOAx9m) has been created, allowing public access to the vapA analyses and relevant metadata. In sum, the results generated provide valuable insights into the global population structure of A. salmonicida, particularly in relation to its piscine host spectrum and the geographic distribution of these hosts.

KW - Aquaculture

KW - Bacterial fish pathogenp

KW - Aeromonas salmonicida

KW - VapA

KW - A-layer

KW - Genotyping

KW - Host specificity

U2 - 10.1093/femsle/fnz074

DO - 10.1093/femsle/fnz074

M3 - Journal article

VL - 366

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

SN - 0378-1097

IS - 7

M1 - fnz074

ER -