Biogas upgrading and biochemical production from gas fermentation: impact of microbial community and gas composition

The present study proposes a novel alternative method of the current biogas upgrading techniques by converting CO₂ (in the biogas) into valuable chemicals (e.g., volatile fatty acids) using H₂ as energy source and acetogenic mixed culture as biocatalyst. The influence of thermal treatment (90°C) on the inhibition of the methanogenic archaea and enriching the acetogenic bacteria in different inocula (mesophilic and thermophilic) was initially tested. The most efficient inoculum that achieved the highest performance through the fermentation process was further used to define the optimum H₂/CO₂ gas ratio that secures maximum production yield of chemicals and maximum biogas upgrading efficiency. In addition, 16S rRNA analysis of the microbial community was conducted at the end of the experimental period to target functional microbes. The maximum biogas content (77% (v/v)) and acetate yield (72%) were achieved for 2H₂:1CO₂ ratio (v/v), with Moorella sp. 4 as the most dominant thermophilic acetogenic bacterium.