Biofilm formation is not a prerequisite for production of the antibacterial compound tropodithietic acid in Phaeobacter inhibens DSM17395

María Jesús Prol García, Paul D'Alvise, Anita Mac Rygaard, Lone Gram

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Aims
The goal of this study was to investigate if biofilm formation on population level is a physiological requirement for antagonism in Phaeobacter inhibens DSM17395, since the antibiotic compound tropodithietic acid (TDA) is produced by several Roseobacter clade species during growth as multicellular aggregates or biofilms at the air–liquid interface and is induced on single cell level upon attachment.
Methods and Results
A mutant library was created by Tn5 transposon insertion and 22 TDA-positive (brown) mutants with decreased biofilm formation or adhesion, and eight TDA-negative (white) mutants with increased biofilm formation or adhesion were selected. None of the selected biofilm-overproducing white mutants showed any antibiotic activity, while all brown mutants with reduced or disabled biofilm formation produced the antibacterial compound. Sequencing analysis indicated that genes that are likely involved in EPS/LPS production, motility and chemotaxis, and redox regulation play a role in biofilm formation and/or adhesion in P. inhibens DSM17395.
Conclusions
Cell aggregation and biofilm formation are not physiological prerequisites for TDA production.
Significance and Impact of the Study
This study contributes to the understanding of TDA production in P. inhibens, which has great potential as a probiotic in marine larviculture
Original languageEnglish
JournalJournal of Applied Microbiology
Volume117
Issue number6
Pages (from-to)1592-1600
ISSN1364-5072
DOIs
Publication statusPublished - 2014

Cite this

@article{fa99953a61c5409791933ce5b90d96ef,
title = "Biofilm formation is not a prerequisite for production of the antibacterial compound tropodithietic acid in Phaeobacter inhibens DSM17395",
abstract = "Aims The goal of this study was to investigate if biofilm formation on population level is a physiological requirement for antagonism in Phaeobacter inhibens DSM17395, since the antibiotic compound tropodithietic acid (TDA) is produced by several Roseobacter clade species during growth as multicellular aggregates or biofilms at the air–liquid interface and is induced on single cell level upon attachment. Methods and Results A mutant library was created by Tn5 transposon insertion and 22 TDA-positive (brown) mutants with decreased biofilm formation or adhesion, and eight TDA-negative (white) mutants with increased biofilm formation or adhesion were selected. None of the selected biofilm-overproducing white mutants showed any antibiotic activity, while all brown mutants with reduced or disabled biofilm formation produced the antibacterial compound. Sequencing analysis indicated that genes that are likely involved in EPS/LPS production, motility and chemotaxis, and redox regulation play a role in biofilm formation and/or adhesion in P. inhibens DSM17395. Conclusions Cell aggregation and biofilm formation are not physiological prerequisites for TDA production. Significance and Impact of the Study This study contributes to the understanding of TDA production in P. inhibens, which has great potential as a probiotic in marine larviculture",
author = "{Prol Garc{\'i}a}, {Mar{\'i}a Jes{\'u}s} and Paul D'Alvise and Rygaard, {Anita Mac} and Lone Gram",
year = "2014",
doi = "10.1111/jam.12659",
language = "English",
volume = "117",
pages = "1592--1600",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell",
number = "6",

}

Biofilm formation is not a prerequisite for production of the antibacterial compound tropodithietic acid in Phaeobacter inhibens DSM17395. / Prol García, María Jesús; D'Alvise, Paul; Rygaard, Anita Mac; Gram, Lone.

In: Journal of Applied Microbiology, Vol. 117, No. 6, 2014, p. 1592-1600.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Biofilm formation is not a prerequisite for production of the antibacterial compound tropodithietic acid in Phaeobacter inhibens DSM17395

AU - Prol García, María Jesús

AU - D'Alvise, Paul

AU - Rygaard, Anita Mac

AU - Gram, Lone

PY - 2014

Y1 - 2014

N2 - Aims The goal of this study was to investigate if biofilm formation on population level is a physiological requirement for antagonism in Phaeobacter inhibens DSM17395, since the antibiotic compound tropodithietic acid (TDA) is produced by several Roseobacter clade species during growth as multicellular aggregates or biofilms at the air–liquid interface and is induced on single cell level upon attachment. Methods and Results A mutant library was created by Tn5 transposon insertion and 22 TDA-positive (brown) mutants with decreased biofilm formation or adhesion, and eight TDA-negative (white) mutants with increased biofilm formation or adhesion were selected. None of the selected biofilm-overproducing white mutants showed any antibiotic activity, while all brown mutants with reduced or disabled biofilm formation produced the antibacterial compound. Sequencing analysis indicated that genes that are likely involved in EPS/LPS production, motility and chemotaxis, and redox regulation play a role in biofilm formation and/or adhesion in P. inhibens DSM17395. Conclusions Cell aggregation and biofilm formation are not physiological prerequisites for TDA production. Significance and Impact of the Study This study contributes to the understanding of TDA production in P. inhibens, which has great potential as a probiotic in marine larviculture

AB - Aims The goal of this study was to investigate if biofilm formation on population level is a physiological requirement for antagonism in Phaeobacter inhibens DSM17395, since the antibiotic compound tropodithietic acid (TDA) is produced by several Roseobacter clade species during growth as multicellular aggregates or biofilms at the air–liquid interface and is induced on single cell level upon attachment. Methods and Results A mutant library was created by Tn5 transposon insertion and 22 TDA-positive (brown) mutants with decreased biofilm formation or adhesion, and eight TDA-negative (white) mutants with increased biofilm formation or adhesion were selected. None of the selected biofilm-overproducing white mutants showed any antibiotic activity, while all brown mutants with reduced or disabled biofilm formation produced the antibacterial compound. Sequencing analysis indicated that genes that are likely involved in EPS/LPS production, motility and chemotaxis, and redox regulation play a role in biofilm formation and/or adhesion in P. inhibens DSM17395. Conclusions Cell aggregation and biofilm formation are not physiological prerequisites for TDA production. Significance and Impact of the Study This study contributes to the understanding of TDA production in P. inhibens, which has great potential as a probiotic in marine larviculture

U2 - 10.1111/jam.12659

DO - 10.1111/jam.12659

M3 - Journal article

C2 - 25284322

VL - 117

SP - 1592

EP - 1600

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 6

ER -