Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants

M. Klausen, Arne Heydorn, Paula Cornelia Ragas, Lotte Munch Lambertsen, A. Aaes-Jorgensen, Søren Molin, Tim Tolker-Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Biofilm formation by Gfp-tagged Pseudomonas aeruginosa PAO1 wild type, flagella and type IV pili mutants in flow chambers irrigated with citrate minimal medium was characterized by the use of confocal laser scanning microscopy and comstat image analysis. Flagella and type IV pili were not necessary for P. aeruginosa initial attachment or biofilm formation, but the cell appendages had roles in biofilm development, as wild type, flagella and type IV pili mutants formed biofilms with different structures. Dynamics and selection during biofilm formation were investigated by tagging the wild type and flagella/type IV mutants with Yfp and Cfp and performing time-lapse confocal laser scanning microscopy in mixed colour biofilms. The initial microcolony formation occurred by clonal growth, after which wild-type P. aeruginosa bacteria spread over the substratum by means of twitching motility. The wild-type biofilms were dynamic compositions with extensive motility, competition and selection occurring during development. Bacterial migration prevented the formation of larger microcolonial structures in the wild-type biofilms. The results are discussed in relation to the current model for P. aeruginosa biofilm development.
    Original languageEnglish
    JournalMolecular Microbiology
    Volume48
    Issue number6
    Pages (from-to)1511-1524
    ISSN0950-382X
    Publication statusPublished - 2003

    Cite this

    Klausen, M., Heydorn, A., Ragas, P. C., Lambertsen, L. M., Aaes-Jorgensen, A., Molin, S., & Tolker-Nielsen, T. (2003). Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants. Molecular Microbiology, 48(6), 1511-1524.