Bioelectrochemical probing of intracellular redox processes in living yeast cells—application of redox polymer wiring in a microfluidic environment

Arto Heiskanen, Vasile Coman, Natalie Kostesha, David Sabourin, Nick Haslett, Keith Baronian, Lo Gorton, Martin Dufva, Jenny Emnéus

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Conventionally, microbial bioelectrochemical assays have been conducted using immobilized cells on an electrode that is placed in an electrochemical batch cell. In this paper, we describe a developed microfluidic platform with integrated microelectrode arrays for automated bioelectrochemical assays utilizing a new double mediator system to map redox metabolism and screen for genetic modifications in Saccharomyces cerevisiae cells. The function of this new double mediator system based on menadione and osmium redox polymer (PVI-Os) is demonstrated. “Wiring” of S. cerevisiae cells using PVI-Os shows a significant improvement of bioelectrochemical monitoring in a microfluidic environment and functions as an effective immobilization matrix for cells that are not strongly adherent. The function of the developed microfluidic platform is demonstrated using two strains of S. cerevisiae, ENY.WA and its deletion mutant EBY44, which lacks the enzyme phosphoglucose isomerase. The cellular responses to introduced glucose and fructose were recorded for the two S. cerevisiae strains, and the obtained results are compared with previously published work when using an electrochemical batch cell, indicating that microfluidic bioelectrochemical assays employing the menadione–PVI-Os double mediator system provides an effective means to conduct automated microbial assays. FigureMicrofluidic platform for bioelectrochemical assays using osmium redox polymer “wired” living yeast cells
    Original languageEnglish
    JournalAnalytical and Bioanalytical Chemistry
    Volume405
    Issue number11
    Pages (from-to)3847-3858
    ISSN1618-2642
    DOIs
    Publication statusPublished - 2013

    Keywords

    • Cellular redox activity
    • Microbial bioelectrochemistry
    • Osmium redox polymer
    • Osmium redox polymer system
    • Saccharomyces cerevisiae
    • Microfluidic system

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