Bioelectrocatalytic reduction of oxygen at gold nanoparticles modified with laccase

Vida Krikstolaityte; Alejandro Barrantes; Arunas Ramanavicius; Thomas Arnebrant; Sergey Shleev; Tautgirdas Ruzgas

doi:10.1016/j.bioelechem.2013.09.004

Bioelectrocatalytic reduction of oxygen at gold nanoparticles modified with laccase

Vida Krikstolaityte, Alejandro Barrantes, Arunas Ramanavicius, Thomas Arnebrant, Sergey Shleev, Tautgirdas Ruzgas

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

To characterise bioelectrocatalytic oxygen reduction at gold nanoparticles (AuNPs) modified with Trametes hirsuta laccase (ThLc) combined electrochemical and quartz crystal microbalance measurements have been used. The electrodes with different degrees of AuNP-monolayer coverage, θ, have been studied. In every case of θ close to theoretically possible 44 ThLc molecules adsorbed at 22nm diameter AuNP. The bioelectrocatalytic current was recalculated down to the current at a single AuNP. Unexpectedly, the current at a single AuNP was higher when θ was higher. The maximum current reached at a single AuNP was 31·10^-18 A which corresponds to the enzyme turnover (k_cat) 13s^-1. This rate is lower than the homogeneous ThLc turnover (190s^-1) suggesting partial denaturation of ThLc upon adsorption or that some ThLc are not in DET contact with the electrode surface. © 2013 Elsevier B.V.

Original language	English
Journal	Bioelectrochemistry
Volume	95
Pages (from-to)	1-6
Number of pages	6
ISSN	1567-5394
DOIs	https://doi.org/10.1016/j.bioelechem.2013.09.004
Publication status	Published - 2014
Externally published	Yes

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title = "Bioelectrocatalytic reduction of oxygen at gold nanoparticles modified with laccase",

abstract = "To characterise bioelectrocatalytic oxygen reduction at gold nanoparticles (AuNPs) modified with Trametes hirsuta laccase (ThLc) combined electrochemical and quartz crystal microbalance measurements have been used. The electrodes with different degrees of AuNP-monolayer coverage, θ, have been studied. In every case of θ close to theoretically possible 44 ThLc molecules adsorbed at 22nm diameter AuNP. The bioelectrocatalytic current was recalculated down to the current at a single AuNP. Unexpectedly, the current at a single AuNP was higher when θ was higher. The maximum current reached at a single AuNP was 31·10-18 A which corresponds to the enzyme turnover (kcat) 13s-1. This rate is lower than the homogeneous ThLc turnover (190s-1) suggesting partial denaturation of ThLc upon adsorption or that some ThLc are not in DET contact with the electrode surface. {\textcopyright} 2013 Elsevier B.V.",

author = "Vida Krikstolaityte and Alejandro Barrantes and Arunas Ramanavicius and Thomas Arnebrant and Sergey Shleev and Tautgirdas Ruzgas",

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doi = "10.1016/j.bioelechem.2013.09.004",

language = "English",

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journal = "Bioelectrochemistry",

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TY - JOUR

T1 - Bioelectrocatalytic reduction of oxygen at gold nanoparticles modified with laccase

AU - Krikstolaityte, Vida

AU - Barrantes, Alejandro

AU - Ramanavicius, Arunas

AU - Arnebrant, Thomas

AU - Shleev, Sergey

AU - Ruzgas, Tautgirdas

PY - 2014

Y1 - 2014

N2 - To characterise bioelectrocatalytic oxygen reduction at gold nanoparticles (AuNPs) modified with Trametes hirsuta laccase (ThLc) combined electrochemical and quartz crystal microbalance measurements have been used. The electrodes with different degrees of AuNP-monolayer coverage, θ, have been studied. In every case of θ close to theoretically possible 44 ThLc molecules adsorbed at 22nm diameter AuNP. The bioelectrocatalytic current was recalculated down to the current at a single AuNP. Unexpectedly, the current at a single AuNP was higher when θ was higher. The maximum current reached at a single AuNP was 31·10-18 A which corresponds to the enzyme turnover (kcat) 13s-1. This rate is lower than the homogeneous ThLc turnover (190s-1) suggesting partial denaturation of ThLc upon adsorption or that some ThLc are not in DET contact with the electrode surface. © 2013 Elsevier B.V.

AB - To characterise bioelectrocatalytic oxygen reduction at gold nanoparticles (AuNPs) modified with Trametes hirsuta laccase (ThLc) combined electrochemical and quartz crystal microbalance measurements have been used. The electrodes with different degrees of AuNP-monolayer coverage, θ, have been studied. In every case of θ close to theoretically possible 44 ThLc molecules adsorbed at 22nm diameter AuNP. The bioelectrocatalytic current was recalculated down to the current at a single AuNP. Unexpectedly, the current at a single AuNP was higher when θ was higher. The maximum current reached at a single AuNP was 31·10-18 A which corresponds to the enzyme turnover (kcat) 13s-1. This rate is lower than the homogeneous ThLc turnover (190s-1) suggesting partial denaturation of ThLc upon adsorption or that some ThLc are not in DET contact with the electrode surface. © 2013 Elsevier B.V.

U2 - 10.1016/j.bioelechem.2013.09.004

DO - 10.1016/j.bioelechem.2013.09.004

M3 - Journal article

C2 - 24134999

SN - 1567-5394

VL - 95

SP - 1

EP - 6

JO - Bioelectrochemistry

JF - Bioelectrochemistry

ER -

Bioelectrocatalytic reduction of oxygen at gold nanoparticles modified with laccase

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