Biodiversity of soil bacteria exposed to sub-lethal concentrations of phosphonium-based ionic liquids: Effects of toxicity and biodegradation

Mateusz Sydow, Mikołaj Owsianiak, Grzegorz Framski, Marta Woźniak-Karczewska, Agnieszka Piotrowska-Cyplik, Łukasz Ławniczak, Alicja Szulc, Agnieszka Zgoła-Grześkowiak, Hermann J Heipieper, Łukasz Chrzanowski*

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    Little is known about the effect of ionic liquids (ILs) on the structure of soil microbial communities and resulting biodiversity. Therefore, we studied the influence of six trihexyl(tetradecyl)phosphonium ILs (with either bromide or various organic anions) at sublethal concentrations on the structure of microbial community present in an urban park soil in 100-day microcosm experiments. The biodiversity decreased in all samples (Shannon's index decreased from 1.75 down to 0.74 and OTU's number decreased from 1399 down to 965) with the largest decrease observed in the microcosms spiked with ILs where biodegradation extent was higher than 80%. (i.e. [P66614][Br] and [P66614][2,4,4]). Despite this general decrease in biodiversity, which can be explained by ecotoxic effect of the ILs, the microbial community in the microcosms was enriched with Gram-negative hydrocarbon-degrading genera e.g. Sphingomonas. It is hypothesized that, in addition to toxicity, the observed decrease in biodiversity and change in the microbial community structure may be explained by the primary biodegradation of the ILs or their metabolites by the mentioned genera, which outcompeted other microorganisms unable to degrade ILs or their metabolites. Thus, the introduction of phosphonium-based ILs into soils at sub-lethal concentrations may result not only in a decrease in biodiversity due to toxic effects, but also in enrichment with ILs-degrading bacteria.
    Original languageEnglish
    JournalEcotoxicology and Environmental Safety
    Volume147
    Pages (from-to)157-164
    ISSN0147-6513
    DOIs
    Publication statusPublished - 2018

    Keywords

    • Biodegradation
    • Biodiversity
    • Illumina NGS
    • Ionic liquids
    • Microbial community
    • Toxicity

    Cite this

    Sydow, Mateusz ; Owsianiak, Mikołaj ; Framski, Grzegorz ; Woźniak-Karczewska, Marta ; Piotrowska-Cyplik, Agnieszka ; Ławniczak, Łukasz ; Szulc, Alicja ; Zgoła-Grześkowiak, Agnieszka ; Heipieper, Hermann J ; Chrzanowski, Łukasz. / Biodiversity of soil bacteria exposed to sub-lethal concentrations of phosphonium-based ionic liquids: Effects of toxicity and biodegradation. In: Ecotoxicology and Environmental Safety. 2018 ; Vol. 147. pp. 157-164.
    @article{f174cbd39c774c63ae6b8ce97cc72f2a,
    title = "Biodiversity of soil bacteria exposed to sub-lethal concentrations of phosphonium-based ionic liquids: Effects of toxicity and biodegradation",
    abstract = "Little is known about the effect of ionic liquids (ILs) on the structure of soil microbial communities and resulting biodiversity. Therefore, we studied the influence of six trihexyl(tetradecyl)phosphonium ILs (with either bromide or various organic anions) at sublethal concentrations on the structure of microbial community present in an urban park soil in 100-day microcosm experiments. The biodiversity decreased in all samples (Shannon's index decreased from 1.75 down to 0.74 and OTU's number decreased from 1399 down to 965) with the largest decrease observed in the microcosms spiked with ILs where biodegradation extent was higher than 80{\%}. (i.e. [P66614][Br] and [P66614][2,4,4]). Despite this general decrease in biodiversity, which can be explained by ecotoxic effect of the ILs, the microbial community in the microcosms was enriched with Gram-negative hydrocarbon-degrading genera e.g. Sphingomonas. It is hypothesized that, in addition to toxicity, the observed decrease in biodiversity and change in the microbial community structure may be explained by the primary biodegradation of the ILs or their metabolites by the mentioned genera, which outcompeted other microorganisms unable to degrade ILs or their metabolites. Thus, the introduction of phosphonium-based ILs into soils at sub-lethal concentrations may result not only in a decrease in biodiversity due to toxic effects, but also in enrichment with ILs-degrading bacteria.",
    keywords = "Biodegradation, Biodiversity, Illumina NGS, Ionic liquids, Microbial community, Toxicity",
    author = "Mateusz Sydow and Mikołaj Owsianiak and Grzegorz Framski and Marta Woźniak-Karczewska and Agnieszka Piotrowska-Cyplik and Łukasz Ławniczak and Alicja Szulc and Agnieszka Zgoła-Grześkowiak and Heipieper, {Hermann J} and Łukasz Chrzanowski",
    year = "2018",
    doi = "10.1016/j.ecoenv.2017.08.026",
    language = "English",
    volume = "147",
    pages = "157--164",
    journal = "Ecotoxicology and Environmental Safety",
    issn = "0147-6513",
    publisher = "Academic Press",

    }

    Sydow, M, Owsianiak, M, Framski, G, Woźniak-Karczewska, M, Piotrowska-Cyplik, A, Ławniczak, Ł, Szulc, A, Zgoła-Grześkowiak, A, Heipieper, HJ & Chrzanowski, Ł 2018, 'Biodiversity of soil bacteria exposed to sub-lethal concentrations of phosphonium-based ionic liquids: Effects of toxicity and biodegradation', Ecotoxicology and Environmental Safety, vol. 147, pp. 157-164. https://doi.org/10.1016/j.ecoenv.2017.08.026

    Biodiversity of soil bacteria exposed to sub-lethal concentrations of phosphonium-based ionic liquids: Effects of toxicity and biodegradation. / Sydow, Mateusz; Owsianiak, Mikołaj; Framski, Grzegorz; Woźniak-Karczewska, Marta; Piotrowska-Cyplik, Agnieszka; Ławniczak, Łukasz; Szulc, Alicja; Zgoła-Grześkowiak, Agnieszka; Heipieper, Hermann J; Chrzanowski, Łukasz.

    In: Ecotoxicology and Environmental Safety, Vol. 147, 2018, p. 157-164.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Biodiversity of soil bacteria exposed to sub-lethal concentrations of phosphonium-based ionic liquids: Effects of toxicity and biodegradation

    AU - Sydow, Mateusz

    AU - Owsianiak, Mikołaj

    AU - Framski, Grzegorz

    AU - Woźniak-Karczewska, Marta

    AU - Piotrowska-Cyplik, Agnieszka

    AU - Ławniczak, Łukasz

    AU - Szulc, Alicja

    AU - Zgoła-Grześkowiak, Agnieszka

    AU - Heipieper, Hermann J

    AU - Chrzanowski, Łukasz

    PY - 2018

    Y1 - 2018

    N2 - Little is known about the effect of ionic liquids (ILs) on the structure of soil microbial communities and resulting biodiversity. Therefore, we studied the influence of six trihexyl(tetradecyl)phosphonium ILs (with either bromide or various organic anions) at sublethal concentrations on the structure of microbial community present in an urban park soil in 100-day microcosm experiments. The biodiversity decreased in all samples (Shannon's index decreased from 1.75 down to 0.74 and OTU's number decreased from 1399 down to 965) with the largest decrease observed in the microcosms spiked with ILs where biodegradation extent was higher than 80%. (i.e. [P66614][Br] and [P66614][2,4,4]). Despite this general decrease in biodiversity, which can be explained by ecotoxic effect of the ILs, the microbial community in the microcosms was enriched with Gram-negative hydrocarbon-degrading genera e.g. Sphingomonas. It is hypothesized that, in addition to toxicity, the observed decrease in biodiversity and change in the microbial community structure may be explained by the primary biodegradation of the ILs or their metabolites by the mentioned genera, which outcompeted other microorganisms unable to degrade ILs or their metabolites. Thus, the introduction of phosphonium-based ILs into soils at sub-lethal concentrations may result not only in a decrease in biodiversity due to toxic effects, but also in enrichment with ILs-degrading bacteria.

    AB - Little is known about the effect of ionic liquids (ILs) on the structure of soil microbial communities and resulting biodiversity. Therefore, we studied the influence of six trihexyl(tetradecyl)phosphonium ILs (with either bromide or various organic anions) at sublethal concentrations on the structure of microbial community present in an urban park soil in 100-day microcosm experiments. The biodiversity decreased in all samples (Shannon's index decreased from 1.75 down to 0.74 and OTU's number decreased from 1399 down to 965) with the largest decrease observed in the microcosms spiked with ILs where biodegradation extent was higher than 80%. (i.e. [P66614][Br] and [P66614][2,4,4]). Despite this general decrease in biodiversity, which can be explained by ecotoxic effect of the ILs, the microbial community in the microcosms was enriched with Gram-negative hydrocarbon-degrading genera e.g. Sphingomonas. It is hypothesized that, in addition to toxicity, the observed decrease in biodiversity and change in the microbial community structure may be explained by the primary biodegradation of the ILs or their metabolites by the mentioned genera, which outcompeted other microorganisms unable to degrade ILs or their metabolites. Thus, the introduction of phosphonium-based ILs into soils at sub-lethal concentrations may result not only in a decrease in biodiversity due to toxic effects, but also in enrichment with ILs-degrading bacteria.

    KW - Biodegradation

    KW - Biodiversity

    KW - Illumina NGS

    KW - Ionic liquids

    KW - Microbial community

    KW - Toxicity

    U2 - 10.1016/j.ecoenv.2017.08.026

    DO - 10.1016/j.ecoenv.2017.08.026

    M3 - Journal article

    VL - 147

    SP - 157

    EP - 164

    JO - Ecotoxicology and Environmental Safety

    JF - Ecotoxicology and Environmental Safety

    SN - 0147-6513

    ER -