Biodegradable hyaluronic acid/N-carboxyethyl chitosan/protein ternary complexes as implantable carriers for controlled protein release

Hongliang Jiang; Yijuan Wang; Qian Huang; Yan Li; Chaonan Xu; Kangjie Zhu; Weiliam Chen

doi:10.1002/mabi.200500126

Biodegradable hyaluronic acid/N-carboxyethyl chitosan/protein ternary complexes as implantable carriers for controlled protein release

Hongliang Jiang, Yijuan Wang, Qian Huang, Yan Li, Chaonan Xu, Kangjie Zhu, Weiliam Chen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

An ampholytic N-carboxyethyl chitosan (CEC), with various isoelectric points (IPs), was synthesized by grafting acrylic acid on chitosan utilizing Michael's reaction. Compared to native chitosan, CEC has enhanced water solubility and dramatically accelerated enzymatic degradation; the rate of degradation is proportional to the degree of substitution (DS). The results from turbidimetric titration and fluorescence studies revealed that CEC formed complexes with either hyaluronic acid (HA) or bovine serum albumin (BSA) within a certain pH range. The HA/CEC/BSA ternary complexes could be prepared by colloid titration with quantitative yield and BSA entrapment. The rate of BSA release from the complexes was affected by pH, ionic strength, DS of CEC, and the molecular weight (MW) of HA. The endurance of BSA release from the complexes could be extended up to 20 d by formulating them with high-MW HA and CEC with low DS.
Keyword: controlled release; hyaluronic acid; N-carboxyethyl chitosan; proteins; ternary complex

Original language	English
Journal	Macromolecular Bioscience
Volume	5
Issue number	12
Pages (from-to)	1226-1233
ISSN	1616-5187
DOIs	https://doi.org/10.1002/mabi.200500126
Publication status	Published - 2005
Externally published	Yes

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title = "Biodegradable hyaluronic acid/N-carboxyethyl chitosan/protein ternary complexes as implantable carriers for controlled protein release",

abstract = "An ampholytic N-carboxyethyl chitosan (CEC), with various isoelectric points (IPs), was synthesized by grafting acrylic acid on chitosan utilizing Michael's reaction. Compared to native chitosan, CEC has enhanced water solubility and dramatically accelerated enzymatic degradation; the rate of degradation is proportional to the degree of substitution (DS). The results from turbidimetric titration and fluorescence studies revealed that CEC formed complexes with either hyaluronic acid (HA) or bovine serum albumin (BSA) within a certain pH range. The HA/CEC/BSA ternary complexes could be prepared by colloid titration with quantitative yield and BSA entrapment. The rate of BSA release from the complexes was affected by pH, ionic strength, DS of CEC, and the molecular weight (MW) of HA. The endurance of BSA release from the complexes could be extended up to 20 d by formulating them with high-MW HA and CEC with low DS. Keyword: controlled release; hyaluronic acid; N-carboxyethyl chitosan; proteins; ternary complex",

author = "Hongliang Jiang and Yijuan Wang and Qian Huang and Yan Li and Chaonan Xu and Kangjie Zhu and Weiliam Chen",

year = "2005",

doi = "10.1002/mabi.200500126",

language = "English",

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journal = "Macromolecular Bioscience",

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TY - JOUR

T1 - Biodegradable hyaluronic acid/N-carboxyethyl chitosan/protein ternary complexes as implantable carriers for controlled protein release

AU - Jiang, Hongliang

AU - Wang, Yijuan

AU - Huang, Qian

AU - Li, Yan

AU - Xu, Chaonan

AU - Zhu, Kangjie

AU - Chen, Weiliam

PY - 2005

Y1 - 2005

N2 - An ampholytic N-carboxyethyl chitosan (CEC), with various isoelectric points (IPs), was synthesized by grafting acrylic acid on chitosan utilizing Michael's reaction. Compared to native chitosan, CEC has enhanced water solubility and dramatically accelerated enzymatic degradation; the rate of degradation is proportional to the degree of substitution (DS). The results from turbidimetric titration and fluorescence studies revealed that CEC formed complexes with either hyaluronic acid (HA) or bovine serum albumin (BSA) within a certain pH range. The HA/CEC/BSA ternary complexes could be prepared by colloid titration with quantitative yield and BSA entrapment. The rate of BSA release from the complexes was affected by pH, ionic strength, DS of CEC, and the molecular weight (MW) of HA. The endurance of BSA release from the complexes could be extended up to 20 d by formulating them with high-MW HA and CEC with low DS. Keyword: controlled release; hyaluronic acid; N-carboxyethyl chitosan; proteins; ternary complex

AB - An ampholytic N-carboxyethyl chitosan (CEC), with various isoelectric points (IPs), was synthesized by grafting acrylic acid on chitosan utilizing Michael's reaction. Compared to native chitosan, CEC has enhanced water solubility and dramatically accelerated enzymatic degradation; the rate of degradation is proportional to the degree of substitution (DS). The results from turbidimetric titration and fluorescence studies revealed that CEC formed complexes with either hyaluronic acid (HA) or bovine serum albumin (BSA) within a certain pH range. The HA/CEC/BSA ternary complexes could be prepared by colloid titration with quantitative yield and BSA entrapment. The rate of BSA release from the complexes was affected by pH, ionic strength, DS of CEC, and the molecular weight (MW) of HA. The endurance of BSA release from the complexes could be extended up to 20 d by formulating them with high-MW HA and CEC with low DS. Keyword: controlled release; hyaluronic acid; N-carboxyethyl chitosan; proteins; ternary complex

U2 - 10.1002/mabi.200500126

DO - 10.1002/mabi.200500126

M3 - Journal article

C2 - 16307434

SN - 1616-5187

VL - 5

SP - 1226

EP - 1233

JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

IS - 12

ER -

Biodegradable hyaluronic acid/N-carboxyethyl chitosan/protein ternary complexes as implantable carriers for controlled protein release

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