Biodegradable hyaluronic acid/N-carboxyethyl chitosan/protein ternary complexes as implantable carriers for controlled protein release

Hongliang Jiang, Yijuan Wang, Qian Huang, Yan Li, Chaonan Xu, Kangjie Zhu, Weiliam Chen

Research output: Contribution to journalJournal articleResearchpeer-review


An ampholytic N-carboxyethyl chitosan (CEC), with various isoelectric points (IPs), was synthesized by grafting acrylic acid on chitosan utilizing Michael's reaction. Compared to native chitosan, CEC has enhanced water solubility and dramatically accelerated enzymatic degradation; the rate of degradation is proportional to the degree of substitution (DS). The results from turbidimetric titration and fluorescence studies revealed that CEC formed complexes with either hyaluronic acid (HA) or bovine serum albumin (BSA) within a certain pH range. The HA/CEC/BSA ternary complexes could be prepared by colloid titration with quantitative yield and BSA entrapment. The rate of BSA release from the complexes was affected by pH, ionic strength, DS of CEC, and the molecular weight (MW) of HA. The endurance of BSA release from the complexes could be extended up to 20 d by formulating them with high-MW HA and CEC with low DS.
Keyword: controlled release; hyaluronic acid; N-carboxyethyl chitosan; proteins; ternary complex
Original languageEnglish
JournalMacromolecular Bioscience
Issue number12
Pages (from-to)1226-1233
Publication statusPublished - 2005
Externally publishedYes

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