Biodecolorization of Azo Dye Remazol Orange by Pseudomonas aeruginosa BCH and Toxicity (Oxidative Stress) Reduction in Allium cepa Root Cells

Shekhar B. Jadhav, Shripad N. Surwase, Dayanand Kalyani, Ranjit G. Gurav, Jyoti P. Jadhav

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In this report a textile azo dye Remazol orange was degraded and detoxified by bacterium Pseudomonas aeruginosa BCH in plain distilled water. This bacterial decolorization performance was found to be pH and temperature dependent with maximum decolorization observed at pH 8 and temperature 30 °C. Bacterium tolerated higher dye concentrations up to 400 mg l−1. Effect of initial cell mass showed that higher cell mass concentration can accelerate decolorization process with maximum of 92 % decolorization observed at 2.5 g l−1 cell mass within 6.5 h. Effect of various metal ions showed Mn has inducing effect whereas Zn strongly inhibited the decolorization process at 5 mM concentration. Analysis of biodegradation products carried out with UV–vis spectroscopy, HPTLC and FTIR confirmed the decolorization and degradation of Remazol orange. Possible route for the degradation of dye was proposed based on GC-MS analysis. During toxicological scrutiny in Allium cepa root cells, induction in the activities of superoxide dismutase (SOD), guaiacol peroxidase (GPX) and inhibition of catalase (CAT) along with raised levels of lipid peroxidation and protein oxidation in dye treated samples were detected which conclusively indicated the generation of oxidative stress. Less toxic nature of the dye degraded products was observed after bacterial treatment.
Original languageEnglish
JournalApplied Biochemistry and Biotechnology
Volume168
Issue number5
Pages (from-to)1319-1334
ISSN0273-2289
DOIs
Publication statusPublished - 2012
Externally publishedYes

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