@inbook{592dd176692a465187570abd3fc37e03,
title = "Bioconversion of phytosterols into androstadienedione by Mycobacterium smegmatis CECT 8331",
abstract = "The C19 steroid 1,4-androstadiene-3,17-dione (androstadienedione, ADD) is an added value product used as a synthon in the pharmaceutical industry for the commercial production of corticosteroids, mineralocorticoids, oral contraceptives, and other pharmaceutical steroids. Phytosterol biotransformation catalyzed by microbial whole cells is actually a very well-established research area in white biotechnology. The protocol below provides detailed information on ADD production by the mutant CECT 8331 of Mycobacterium smegmatis mc2155 using phytosterols as raw material in a lab scale. This protocol describes the bioconversion of phytosterols into ADD in a single fermentation step.",
keywords = "ADD, Androstadienedione, Biotransformation, Mycobacterium smegmatis, Phytosterols, Steroids",
author = "Julia Garc{\'i}a-Fern{\'a}ndez and Igor Mart{\'i}nez and Lorena Fern{\'a}ndez-Cabez{\'o}n and Carmen Felpeto-Santero and Garc{\'i}a, \{Jos{\'e} Luis\} and Beatriz Gal{\'a}n",
year = "2017",
doi = "10.1007/978-1-4939-7183-1\_15",
language = "English",
isbn = "978-1-4939-7182-4",
volume = "1645",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "211--225",
editor = "Jos{\'e}-Louis Barredo and Ignacio Herr{\'a}iz",
booktitle = "Microbial Steroids",
address = "United States",
}