Bioconversion of phytosterols into androstadienedione by Mycobacterium smegmatis CECT 8331

Julia García-Fernández, Igor Martí­nez, Lorena Fernández-Cabezón, Carmen Felpeto-Santero, José Luis Garcí­a, Beatriz Galán

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review


The C19 steroid 1,4-androstadiene-3,17-dione (androstadienedione, ADD) is an added value product used as a synthon in the pharmaceutical industry for the commercial production of corticosteroids, mineralocorticoids, oral contraceptives, and other pharmaceutical steroids. Phytosterol biotransformation catalyzed by microbial whole cells is actually a very well-established research area in white biotechnology. The protocol below provides detailed information on ADD production by the mutant CECT 8331 of Mycobacterium smegmatis mc2155 using phytosterols as raw material in a lab scale. This protocol describes the bioconversion of phytosterols into ADD in a single fermentation step.
Original languageEnglish
Title of host publicationMicrobial Steroids
EditorsJosé-Louis Barredo, Ignacio Herráiz
Place of PublicationNew York
PublisherHumana Press
Publication date2017
ISBN (Print)978-1-4939-7182-4
ISBN (Electronic)978-1-4939-7183-1
Publication statusPublished - 2017
Externally publishedYes
SeriesMethods in Molecular Biology


  • ADD
  • Androstadienedione
  • Biotransformation
  • Mycobacterium smegmatis
  • Phytosterols
  • Steroids

Cite this

García-Fernández, J., Martí­nez, I., Fernández-Cabezón, L., Felpeto-Santero, C., Garcí­a, J. L., & Galán, B. (2017). Bioconversion of phytosterols into androstadienedione by Mycobacterium smegmatis CECT 8331. In J-L. Barredo, & I. Herráiz (Eds.), Microbial Steroids (Vol. 1645, pp. 211-225). Humana Press. Methods in Molecular Biology