Biochemical Characterization of CPS-1, a Subclass B3 Metallo-beta-Lactamase from a Chryseobacterium piscium Soil Isolate

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DOI

  • Author: Gudeta, Dereje Dadi

  • Author: Pollini, Simona

  • Author: Docquier, Jean-Denis

  • Author: Bortolaia, Valeria

    University of Copenhagen

  • Author: Rossolini, Gian Maria

  • Author: Guardabassi, Luca

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CPS-1 is a subclass B3 metallo-beta-lactamase from a Chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the GOB-1 enzyme. CPS-1 was overproduced in Escherichia coli Rosetta (DE3), purified by chromatography, and biochemically characterized. This enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which overall resembles those of L1, GOB-1, and acquired subclass B3 enzymes AIM-1 and SMB-1.
Original languageEnglish
JournalAntimicrobial Agents and Chemotherapy
Volume60
Issue number3
Pages (from-to)1869-1873
Number of pages5
ISSN0066-4804
DOIs
Publication statusPublished - 2016
Externally publishedYes
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Pharmacology (medical), Pharmacology, Infectious Diseases, ampicillin, carbamoyl phosphate synthase, cefalotin, cefotaxime, cefoxitin, ceftriaxone, cefuroxime, doripenem, imipenem, meropenem, metallo beta lactamase, penicillin G, ticarcillin, Article, biochemical composition, Bradyrhizobium japonicum, Chryseobacterium, Chryseobacterium formosense, Chryseobacterium piscium, comparative study, controlled study, Elizabethkingia meningoseptica, enzyme specificity, Escherichia coli, Legionella, Legionella gormanii, molecular weight, nonhuman, open reading frame, priority journal, Pseudomonas aeruginosa, Serratia marcescens, soil microflora, Stenotrophomonas maltophilia, Genetics - General, Biochemistry studies - Proteins, peptides and amino acids, Pathology - Therapy, Pharmacology - General, Physiology and biochemistry of bacteria, Genetics of bacteria and viruses, Chemotherapy - General, methods and metabolism, Chemotherapy - Antibacterial agents, penicillin, cephalosporin, carbapenem, metallo-beta-lactamase, Bacteria, Eubacteria, Microorganisms, soil isolate, Mechanisms of Resistance, MICROBIOLOGY, PHARMACOLOGY, PSEUDOMONAS-AERUGINOSA, ESCHERICHIA-COLI, STENOTROPHOMONAS-MALTOPHILIA, CLINICAL ISOLATE, GENE, CLASSIFICATION, PURIFICATION, DIVERSITY, INTEGRON, INSIGHTS
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