Biochemical Characterization of CPS-1, a Subclass B3 Metallo-beta-Lactamase from a Chryseobacterium piscium Soil Isolate

Dereje Dadi Gudeta; Simona Pollini; Jean-Denis Docquier; Valeria Bortolaia; Gian Maria Rossolini; Luca Guardabassi

doi:10.1128/AAC.01924-15

Biochemical Characterization of CPS-1, a Subclass B3 Metallo-beta-Lactamase from a Chryseobacterium piscium Soil Isolate

Dereje Dadi Gudeta, Simona Pollini, Jean-Denis Docquier, Valeria Bortolaia, Gian Maria Rossolini, Luca Guardabassi

University of Copenhagen

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Abstract

CPS-1 is a subclass B3 metallo-beta-lactamase from a Chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the GOB-1 enzyme. CPS-1 was overproduced in Escherichia coli Rosetta (DE3), purified by chromatography, and biochemically characterized. This enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which overall resembles those of L1, GOB-1, and acquired subclass B3 enzymes AIM-1 and SMB-1.

Original language	English
Journal	Antimicrobial Agents and Chemotherapy
Volume	60
Issue number	3
Pages (from-to)	1869-1873
Number of pages	5
ISSN	0066-4804
DOIs	https://doi.org/10.1128/AAC.01924-15
Publication status	Published - 2016
Externally published	Yes

Keywords

Pharmacology (medical)
Pharmacology
Infectious Diseases
ampicillin
carbamoyl phosphate synthase
cefalotin
cefotaxime
cefoxitin
ceftriaxone
cefuroxime
doripenem
imipenem
meropenem
metallo beta lactamase
penicillin G
ticarcillin
Article
biochemical composition
Bradyrhizobium japonicum
Chryseobacterium
Chryseobacterium formosense
Chryseobacterium piscium
comparative study
controlled study
Elizabethkingia meningoseptica
enzyme specificity
Escherichia coli
Legionella
Legionella gormanii
molecular weight
nonhuman
open reading frame
priority journal
Pseudomonas aeruginosa
Serratia marcescens
soil microflora
Stenotrophomonas maltophilia
Genetics - General
Biochemistry studies - Proteins, peptides and amino acids
Pathology - Therapy
Pharmacology - General
Physiology and biochemistry of bacteria
Genetics of bacteria and viruses
Chemotherapy - General, methods and metabolism
Chemotherapy - Antibacterial agents
penicillin
cephalosporin
carbapenem
metallo-beta-lactamase
Bacteria, Eubacteria, Microorganisms
soil isolate
Mechanisms of Resistance
MICROBIOLOGY
PHARMACOLOGY
PSEUDOMONAS-AERUGINOSA
ESCHERICHIA-COLI
STENOTROPHOMONAS-MALTOPHILIA
CLINICAL ISOLATE
GENE
CLASSIFICATION
PURIFICATION
DIVERSITY
INTEGRON
INSIGHTS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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abstract = "CPS-1 is a subclass B3 metallo-beta-lactamase from a Chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the GOB-1 enzyme. CPS-1 was overproduced in Escherichia coli Rosetta (DE3), purified by chromatography, and biochemically characterized. This enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which overall resembles those of L1, GOB-1, and acquired subclass B3 enzymes AIM-1 and SMB-1.",

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author = "Gudeta, {Dereje Dadi} and Simona Pollini and Jean-Denis Docquier and Valeria Bortolaia and Rossolini, {Gian Maria} and Luca Guardabassi",

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pages = "1869--1873",

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AU - Gudeta, Dereje Dadi

AU - Pollini, Simona

AU - Docquier, Jean-Denis

AU - Bortolaia, Valeria

AU - Rossolini, Gian Maria

AU - Guardabassi, Luca

PY - 2016

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N2 - CPS-1 is a subclass B3 metallo-beta-lactamase from a Chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the GOB-1 enzyme. CPS-1 was overproduced in Escherichia coli Rosetta (DE3), purified by chromatography, and biochemically characterized. This enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which overall resembles those of L1, GOB-1, and acquired subclass B3 enzymes AIM-1 and SMB-1.

AB - CPS-1 is a subclass B3 metallo-beta-lactamase from a Chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the GOB-1 enzyme. CPS-1 was overproduced in Escherichia coli Rosetta (DE3), purified by chromatography, and biochemically characterized. This enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which overall resembles those of L1, GOB-1, and acquired subclass B3 enzymes AIM-1 and SMB-1.

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KW - doripenem

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KW - meropenem

KW - metallo beta lactamase

KW - penicillin G

KW - ticarcillin

KW - Article

KW - biochemical composition

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KW - Chryseobacterium

KW - Chryseobacterium formosense

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KW - Legionella

KW - Legionella gormanii

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KW - Chemotherapy - General, methods and metabolism

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KW - carbapenem

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KW - INSIGHTS

U2 - 10.1128/AAC.01924-15

DO - 10.1128/AAC.01924-15

M3 - Journal article

C2 - 26666948

SN - 0066-4804

VL - 60

SP - 1869

EP - 1873

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 3

ER -

Biochemical Characterization of CPS-1, a Subclass B3 Metallo-beta-Lactamase from a Chryseobacterium piscium Soil Isolate

Abstract

Keywords

UN SDGs

Access to Document

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