Biochemical Characterization of CPS-1, a Subclass B3 Metallo-beta-Lactamase from a Chryseobacterium piscium Soil Isolate

Dereje Dadi Gudeta, Simona Pollini, Jean-Denis Docquier, Valeria Bortolaia, Gian Maria Rossolini, Luca Guardabassi

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Abstract

CPS-1 is a subclass B3 metallo-beta-lactamase from a Chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the GOB-1 enzyme. CPS-1 was overproduced in Escherichia coli Rosetta (DE3), purified by chromatography, and biochemically characterized. This enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which overall resembles those of L1, GOB-1, and acquired subclass B3 enzymes AIM-1 and SMB-1.
Original languageEnglish
JournalAntimicrobial Agents and Chemotherapy
Volume60
Issue number3
Pages (from-to)1869-1873
Number of pages5
ISSN0066-4804
DOIs
Publication statusPublished - 2016
Externally publishedYes

Keywords

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases
  • ampicillin
  • carbamoyl phosphate synthase
  • cefalotin
  • cefotaxime
  • cefoxitin
  • ceftriaxone
  • cefuroxime
  • doripenem
  • imipenem
  • meropenem
  • metallo beta lactamase
  • penicillin G
  • ticarcillin
  • Article
  • biochemical composition
  • Bradyrhizobium japonicum
  • Chryseobacterium
  • Chryseobacterium formosense
  • Chryseobacterium piscium
  • comparative study
  • controlled study
  • Elizabethkingia meningoseptica
  • enzyme specificity
  • Escherichia coli
  • Legionella
  • Legionella gormanii
  • molecular weight
  • nonhuman
  • open reading frame
  • priority journal
  • Pseudomonas aeruginosa
  • Serratia marcescens
  • soil microflora
  • Stenotrophomonas maltophilia
  • Genetics - General
  • Biochemistry studies - Proteins, peptides and amino acids
  • Pathology - Therapy
  • Pharmacology - General
  • Physiology and biochemistry of bacteria
  • Genetics of bacteria and viruses
  • Chemotherapy - General, methods and metabolism
  • Chemotherapy - Antibacterial agents
  • penicillin
  • cephalosporin
  • carbapenem
  • metallo-beta-lactamase
  • Bacteria, Eubacteria, Microorganisms
  • soil isolate
  • Mechanisms of Resistance
  • MICROBIOLOGY
  • PHARMACOLOGY
  • PSEUDOMONAS-AERUGINOSA
  • ESCHERICHIA-COLI
  • STENOTROPHOMONAS-MALTOPHILIA
  • CLINICAL ISOLATE
  • GENE
  • CLASSIFICATION
  • PURIFICATION
  • DIVERSITY
  • INTEGRON
  • INSIGHTS

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