TY - JOUR
T1 - Biocatalytic System Made of 3D Chitin, Silica Nanopowder and Horseradish Peroxidase for the Removal of 17α-Ethinylestradiol: Determination of Process Efficiency and Degradation Mechanism
AU - Machałowski, Tomasz
AU - Jankowska, Katarzyna
AU - Bachosz, Karolina
AU - Smułek, Wojciech
AU - Ehrlich, Hermann
AU - Kaczorek, Ewa
AU - Zdarta, Jakub
AU - Jesionowski, Teofil
PY - 2022
Y1 - 2022
N2 - The occurrence of 17α-ethinylestradiol (EE2) in the environment and its
removal have drawn special attention from the scientific community in
recent years, due to its hazardous effects on human and wildlife around
the world. Therefore, the aim of this study was to produce an efficient
enzymatic system for the removal of EE2 from aqueous solutions. For the
first time, commercial silica nanopowder and 3D fibrous chitinous
scaffolds from Aplysina fistularis
marine sponge were used as supports for horseradish peroxidase (HRP)
immobilization. The effect of several process parameters onto the
removal mechanism of EE2 by enzymatic conversion and adsorption of EE2
were investigated here, including system type, pH, temperature and
concentrations of H2O2 and EE2. It was possible to fully remove EE2 from aqueous solutions using system SiO2(HRP)–chitin(HRP)
over a wide investigated pH range (5–9) and temperature ranges (4–45
°C). Moreover, the most suitable process conditions have been determined
at pH 7, temperature 25 °C and H2O2 and EE2 concentrations equaling 2 mM and 1 mg/L, respectively. As determined, it was possible to reuse the nanoSiO2(HRP)–chitin(HRP) system to obtain even 55% EE2 degradation efficiency after five consecutive catalytic cycles.
AB - The occurrence of 17α-ethinylestradiol (EE2) in the environment and its
removal have drawn special attention from the scientific community in
recent years, due to its hazardous effects on human and wildlife around
the world. Therefore, the aim of this study was to produce an efficient
enzymatic system for the removal of EE2 from aqueous solutions. For the
first time, commercial silica nanopowder and 3D fibrous chitinous
scaffolds from Aplysina fistularis
marine sponge were used as supports for horseradish peroxidase (HRP)
immobilization. The effect of several process parameters onto the
removal mechanism of EE2 by enzymatic conversion and adsorption of EE2
were investigated here, including system type, pH, temperature and
concentrations of H2O2 and EE2. It was possible to fully remove EE2 from aqueous solutions using system SiO2(HRP)–chitin(HRP)
over a wide investigated pH range (5–9) and temperature ranges (4–45
°C). Moreover, the most suitable process conditions have been determined
at pH 7, temperature 25 °C and H2O2 and EE2 concentrations equaling 2 mM and 1 mg/L, respectively. As determined, it was possible to reuse the nanoSiO2(HRP)–chitin(HRP) system to obtain even 55% EE2 degradation efficiency after five consecutive catalytic cycles.
KW - Silica nanopowder
KW - Chitin
KW - Enzyme immobilization
KW - Horseradish peroxidase
KW - 17α-ethinylestradiol
KW - Degradation
U2 - 10.3390/molecules27041354
DO - 10.3390/molecules27041354
M3 - Journal article
C2 - 35209143
SN - 1420-3049
VL - 27
JO - Molecules
JF - Molecules
IS - 4
M1 - 1354
ER -
