Biocatalytic process development using microfluidic miniaturized systems

Ulrich Krühne, Søren Heintz, Rolf Hoffmeyer Ringborg, Ines Pereira Rosinha, Pär Tufvesson, Krist V. Gernaey, John M. Woodley

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Abstract

The increasing interest in biocatalytic processes means there is a clear need for a new systematic development paradigm which encompasses both protein engineering and process engineering. This paper argues that through the use of a new microfluidic platform, data can be collected more rapidly and integrated with process modeling, can provide the basis for validating a reduced number of potential processes. The miniaturized platform should use a smaller reagent inventory and make better use of precious biocatalysts. The EC funded BIOINTENSE project will use ω-transaminase based synthesis of chiral amines as a test-bed for assessing the viability of such a high throughput biocatalytic process development, and in this paper, such a vision for the future is presented.
Original languageEnglish
JournalGreen Processing and Synthesis
Volume3
Issue number1
Pages (from-to)23-31
ISSN2191-9542
DOIs
Publication statusPublished - 2014

Keywords

  • Biocatalysis
  • Microfluidics
  • Process development
  • Transaminase

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