Bioavailability is improved by enzymatic modification of the citrus flavonoid hesperidin in humans: A randomized, double-blind, crossover trial.

I.L.F. Nielsen, W.S.S. Chee, Lea Bredsdorff, E. Offord-Cavin, Salka E. Rasmussen, Hanne Frederiksen, M. Enslen, D. Barron, M.N. Horcajada, G. Williamson

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Hesperidin is the predominant polyphenol consumed from citrus fruits and juices. However, hesperidin is proposed to have limited bioavailability due to the rutinoside moiety attached to the flavonoid. The aim of this study was to demonstrate in human subjects that the removal of the rhamnose group to yield the corresponding flavonoid glucoside (i.e., hesperetin-7-glucoside) will improve the bioavailability of the aglycone hesperetin. Healthy volunteers (n = 16) completed the double-blind, randomized, crossover study. Subjects randomly consumed hesperetin equivalents supplied as orange juice with natural hesperidin ("low dose"), orange juice treated with hesperidinase enzyme to yield hesperetin-7-glucoside, and orange juice fortified to obtain 3 times more hesperidin than naturally present ("high dose"). The area under the curve (AUC) for total plasma hesperetin of subjects consuming hesperetin-7-glucoside juice was 2-fold higher than that of subjects consuming the "low" dose hesperidin juice [3.45 +/- 1.27 vs. 1.16 +/- 0.52 mmol/(L(.)h), respectively, P > 0.0001]. The AUC for hesperetin after consuming the hesperetin-7-glucoside juice was improved to the level of the "high" dose hesperidin juice [4.16 +/- 1.50 mmol/(L(.)h)]. The peak plasma concentrations (Cm) of hesperetin were 4-fold higher (2.60 +/- 1.07 mmol/L, P <0.0001) after subjects consumed hesperetin-7-glucoside juice compared with those consuming "low" dose hesperidin juice (0.48 +/- 0.27 mmol/L), and 1.5-fold higher than those consuming "high" dose hesperidin juice (1.05 +/- 0.25 mmol/L. The corresponding T-max was much faster (0.6 +/- 0.1 h, P <0.0001) after subjects consumed hesperetin-7-glucoside juice compared with "low" dose (7.0 +/- 3.0 h) and "high" dose (7.4 +/- 2.0 h) hesperidin juices. The results of this study demonstrated that the bioavailability of hesperidin was modulated by enzymatic conversion to hesperetin-7-glucoside, thus changing the absorption site from the colon to the small intestine. This may affect future interventions concerning the health benefits of citrus flavonoids.
Original languageEnglish
JournalJournal of Nutrition
Volume136
Issue number2
Pages (from-to)404-408
ISSN0022-3166
Publication statusPublished - 2006

Keywords

  • citrus antioxidant
  • randomized crossover study
  • bioavailability
  • hesperetin-7-glucoside
  • hesperidin

Fingerprint Dive into the research topics of 'Bioavailability is improved by enzymatic modification of the citrus flavonoid hesperidin in humans: A randomized, double-blind, crossover trial.'. Together they form a unique fingerprint.

Cite this