Bioavailability and effect of α-Fe₂O₃ nanoparticles on growth, fatty acid composition and morphological indices of Chlorella vulgaris

The wide application of α-Fe₂O₃ nanoparticles (NPs) in different fields has resulted in release and accumulation of these materials into the aquatic ecosystem. Therefore, it is important to understand the potential impact of these NPs on aquatic organisms especially primary producers i.e., microalgae. Present study aimed to investigate the bioavailability and the effect of α-Fe₂O₃ NPs on growth of iron deprived cells of Chlorella vulgaris. Results showed that α-Fe₂O₃ NPs are not available as iron source to support the growth of C. vulgaris. Moreover，α-Fe₂O₃ NPs induced stress condition to C. vulgaris, which were reflected in its growth rates, total lipid contents, fatty acid profile and cell morphology. Specifically, low concentrations of α-Fe₂O₃ NPs (0.1, 0.5, 2.5, 5, 10 mg/L) showed similar growth profile and total lipid contents at both exponential and stationary growth phases. At 50 and 100 mg/L α-Fe₂O₃ NPs concentrations biomass reduced by 41.2% and 83.7% whereas total lipid contents increased by 39.7% and 25.5% respectively at exponential growth phase along with reduction in fatty acids. The results illustrated novel insights into the microalgal interaction with nanoparticles, providing fundamental knowledge for the development of future microalgae ecology and cultivation technology.