Bioaugmentation strategy for overcoming ammonia inhibition during biomethanation of a protein-rich substrate

Hailin Tian, Enrico Mancini, Laura Treu, Irini Angelidaki, Ioannis Fotidis*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

High ammonia levels inhibit anaerobic digestion (AD) process and bioaugmentation with ammonia tolerant methanogenic culture is proposed to alleviate ammonia inhibition. In the current study, hydrogenotrophic Methanoculleus bourgensis was bioaugmented in an ammonia-inhibited continuous reactor fed mainly with microalgae (a protein-rich biomass), at extreme ammonia levels (i.e. 11 g NH4+-N L−1). The results showed 28% increase in methane production immediately after bioaugmentation. Moreover, volatile fatty acids decreased rapidly from more than 5 g L−1 to around 1 g L−1, with a fast reduction in propionate concentration. High throughput 16s rRNA gene sequencing demonstrated that the bioaugmented M. bourgensis doubled its relative abundance after bioaugmentation. “Microbiological domino effect”, triggered by the bioaugmented M. bourgensis establishing a newly efficient community, was proposed as the working mechanism of the successful bioaugmentation. Additionally, a strong aceticlastic methanogenesis was found at the end of the experiment evidenced by the dominant presence of Methanosarcina soligelidi and the low abundance of syntrophic acetate oxidising bacteria at the final period. Overall, for the first time, this study proved the positive effect of bioaugmentation on ammonia inhibition alleviation of the microalgae-dominating fed reactor, paving the way of efficient utilization of other protein-rich substrates in the future.
Original languageEnglish
JournalChemosphere
Volume231
Pages (from-to)415-422
ISSN0045-6535
Publication statusPublished - 2019

Keywords

  • Methane
  • Ammonia inhibition
  • Microbial community
  • Methanoculleus bourgensis
  • Hydrogen partial pressure

Cite this

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title = "Bioaugmentation strategy for overcoming ammonia inhibition during biomethanation of a protein-rich substrate",
abstract = "High ammonia levels inhibit anaerobic digestion (AD) process and bioaugmentation with ammonia tolerant methanogenic culture is proposed to alleviate ammonia inhibition. In the current study, hydrogenotrophic Methanoculleus bourgensis was bioaugmented in an ammonia-inhibited continuous reactor fed mainly with microalgae (a protein-rich biomass), at extreme ammonia levels (i.e. 11 g NH4+-N L−1). The results showed 28{\%} increase in methane production immediately after bioaugmentation. Moreover, volatile fatty acids decreased rapidly from more than 5 g L−1 to around 1 g L−1, with a fast reduction in propionate concentration. High throughput 16s rRNA gene sequencing demonstrated that the bioaugmented M. bourgensis doubled its relative abundance after bioaugmentation. “Microbiological domino effect”, triggered by the bioaugmented M. bourgensis establishing a newly efficient community, was proposed as the working mechanism of the successful bioaugmentation. Additionally, a strong aceticlastic methanogenesis was found at the end of the experiment evidenced by the dominant presence of Methanosarcina soligelidi and the low abundance of syntrophic acetate oxidising bacteria at the final period. Overall, for the first time, this study proved the positive effect of bioaugmentation on ammonia inhibition alleviation of the microalgae-dominating fed reactor, paving the way of efficient utilization of other protein-rich substrates in the future.",
keywords = "Methane, Ammonia inhibition, Microbial community, Methanoculleus bourgensis, Hydrogen partial pressure",
author = "Hailin Tian and Enrico Mancini and Laura Treu and Irini Angelidaki and Ioannis Fotidis",
year = "2019",
language = "English",
volume = "231",
pages = "415--422",
journal = "Chemosphere",
issn = "0045-6535",
publisher = "Pergamon Press",

}

Bioaugmentation strategy for overcoming ammonia inhibition during biomethanation of a protein-rich substrate. / Tian, Hailin; Mancini, Enrico; Treu, Laura; Angelidaki, Irini; Fotidis, Ioannis.

In: Chemosphere, Vol. 231, 2019, p. 415-422.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Bioaugmentation strategy for overcoming ammonia inhibition during biomethanation of a protein-rich substrate

AU - Tian, Hailin

AU - Mancini, Enrico

AU - Treu, Laura

AU - Angelidaki, Irini

AU - Fotidis, Ioannis

PY - 2019

Y1 - 2019

N2 - High ammonia levels inhibit anaerobic digestion (AD) process and bioaugmentation with ammonia tolerant methanogenic culture is proposed to alleviate ammonia inhibition. In the current study, hydrogenotrophic Methanoculleus bourgensis was bioaugmented in an ammonia-inhibited continuous reactor fed mainly with microalgae (a protein-rich biomass), at extreme ammonia levels (i.e. 11 g NH4+-N L−1). The results showed 28% increase in methane production immediately after bioaugmentation. Moreover, volatile fatty acids decreased rapidly from more than 5 g L−1 to around 1 g L−1, with a fast reduction in propionate concentration. High throughput 16s rRNA gene sequencing demonstrated that the bioaugmented M. bourgensis doubled its relative abundance after bioaugmentation. “Microbiological domino effect”, triggered by the bioaugmented M. bourgensis establishing a newly efficient community, was proposed as the working mechanism of the successful bioaugmentation. Additionally, a strong aceticlastic methanogenesis was found at the end of the experiment evidenced by the dominant presence of Methanosarcina soligelidi and the low abundance of syntrophic acetate oxidising bacteria at the final period. Overall, for the first time, this study proved the positive effect of bioaugmentation on ammonia inhibition alleviation of the microalgae-dominating fed reactor, paving the way of efficient utilization of other protein-rich substrates in the future.

AB - High ammonia levels inhibit anaerobic digestion (AD) process and bioaugmentation with ammonia tolerant methanogenic culture is proposed to alleviate ammonia inhibition. In the current study, hydrogenotrophic Methanoculleus bourgensis was bioaugmented in an ammonia-inhibited continuous reactor fed mainly with microalgae (a protein-rich biomass), at extreme ammonia levels (i.e. 11 g NH4+-N L−1). The results showed 28% increase in methane production immediately after bioaugmentation. Moreover, volatile fatty acids decreased rapidly from more than 5 g L−1 to around 1 g L−1, with a fast reduction in propionate concentration. High throughput 16s rRNA gene sequencing demonstrated that the bioaugmented M. bourgensis doubled its relative abundance after bioaugmentation. “Microbiological domino effect”, triggered by the bioaugmented M. bourgensis establishing a newly efficient community, was proposed as the working mechanism of the successful bioaugmentation. Additionally, a strong aceticlastic methanogenesis was found at the end of the experiment evidenced by the dominant presence of Methanosarcina soligelidi and the low abundance of syntrophic acetate oxidising bacteria at the final period. Overall, for the first time, this study proved the positive effect of bioaugmentation on ammonia inhibition alleviation of the microalgae-dominating fed reactor, paving the way of efficient utilization of other protein-rich substrates in the future.

KW - Methane

KW - Ammonia inhibition

KW - Microbial community

KW - Methanoculleus bourgensis

KW - Hydrogen partial pressure

M3 - Journal article

VL - 231

SP - 415

EP - 422

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

ER -