Bile acid binding capacity of fish protein hydrolysates from discard species of the West Mediterranean Sea

Raul Perez-Galvez, Pedro Jesús García Moreno, Rocio Morales-Medina, Antonio Guadix, Emilia M. Guadix

Research output: Contribution to journalJournal articleResearchpeer-review


Fish protein hydrolysates (FPH), produced from the six main discard species from the West Mediterranean Sea (sardine, horse mackerel, axillary seabream, bogue, small-spotted catshark and blue whiting) were tested for their bile acid binding capacity. This capacity is directly linked to the ability to inhibit bile reabsorption in the ileum and therefore to lower cholesterol levels in the bloodstream. From each species, FPH were obtained by three different enzymatic treatments employing two serine endoproteases (subtilisin and trypsin) sequentially or in combination. The results show statistically significant differences among the fish species, attaining interesting average values of bile acid binding capacity for blue whiting (27.32% relative to cholestyramine on an equal protein basis) and horse mackerel (27.42% relative to cholestyramine on an equal protein basis). The enzymatic treatments did not significantly affect the ability of a given species to bind bile acids. These results are similar to other protein sources, such as soy protein or casein, of proven hypocholesterolemic effect. It can be concluded that fish protein hydrolysates from these discard species are suitable as ingredients in the formulation of cholesterol-lowering supplements.
Original languageEnglish
JournalFood & Function
Issue number4
Pages (from-to)1261-1267
Number of pages7
Publication statusPublished - 2015
Externally publishedYes


  • Food Science
  • Amino acids
  • Body fluids
  • Cholesterol
  • Fish
  • Hydrolysis
  • Bile acid bindings
  • Cholesterol levels
  • Cholesterol lowering
  • Enzymatic treatments
  • Fish protein hydrolysate
  • Hypocholesterolemic effect
  • Statistically significant difference
  • West mediterraneans
  • Proteins
  • Equidae
  • Micromesistius poutassou
  • Pagellus acarne
  • Scyliorhinus canicula
  • FOOD

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