beta-naphthoflavone and benzo(a)pyrene treatment affect liver intermediary metabolism and plasma cortisol levels in rainbow trout Oncorhynchus mykiss

Adrian Tintos, Manuel Gesto, Jesus M. Miguez, Jose L. Soengas

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

To assess the effects of the polycyclic aromatic hydrocarbons (PAHs) beta-naphthoflavone (beta-NF) and benzo(a)pyrene (BaP) on liver intermediary metabolism and plasma steroid hormones, immature female rainbow trout (Oncorhynchus mykiss) were intraperitoneally injected (2 mu l g(-1)) with vegetable oil alone (control) or containing beta-NF or BaP (10 mg kg(-1)) and returned to their tanks; 3, 24, and 72 h after injection, 11 fish were sampled from each group. On each sampling time, plasma hormone levels (cortisol and 17 beta-estradiol) and metabolic parameters in plasma (glucose, lactate, and alpha-amino acid levels) and liver (glycogen, glucose, lactate, and alpha-amino acid levels, and HK, GK, PK, LDH, G6Pase, G6PDH, FBPase, GDH, Asp-AT, and HOAD activities) were assessed. Changes described for hormonal systems resulted in an increase in plasma levels of cortisol after 24 and 72 h of treatment with both PAHs whereas no changes were noticed for 17 beta-estradiol levels. Changes in intermediary metabolism described effects in several pathways due to treatment with both PAHs. These changes can be summarized as increased glucose and lactate levels in plasma, and increased glycogenolysis and gluconeogenesis in liver after 24 and 72 h of treatment with both PAHs. Furthermore, beta-NF treatment stimulated amino acid catabolism in liver. These metabolic changes can be associated with increased levels of plasma cortisol, and suggest a different metabolic behavior depending on PAHs. (c) 2007 Elsevier Inc. All rights reserved.
Original languageEnglish
JournalEcotoxicology and Environmental Safety
Volume69
Issue number2
Pages (from-to)180-186
Number of pages7
ISSN0147-6513
DOIs
Publication statusPublished - 2008
Externally publishedYes

Keywords

  • ENVIRONMENTAL
  • TOXICOLOGY
  • ANGUILLA-ANGUILLA L.
  • POLYCYCLIC AROMATIC-HYDROCARBONS
  • FLOUNDER PLATICHTHYS-FLESUS
  • ERYTHROCYTIC NUCLEAR ABNORMALITIES
  • GILTHEAD SEA BREAM
  • ENERGY-METABOLISM
  • BIOTRANSFORMATION ENZYMES
  • CARBOHYDRATE-METABOLISM
  • NAPHTHALENE TREATMENT
  • ENDOCRINE RESPONSES
  • beta-naphthoflavone
  • benzo(a)pyrene
  • cortisol
  • 17 beta-estradiol
  • energy metabolism
  • rainbow trout
  • Amino Acids
  • Animals
  • Aspartate Aminotransferases
  • Benzo(a)pyrene
  • Blood Glucose
  • Estradiol
  • Female
  • Fructose-Bisphosphatase
  • Glucose-6-Phosphatase
  • Glucosephosphate Dehydrogenase
  • Glutamate Dehydrogenase
  • Hydrocortisone
  • Lactic Acid
  • Liver
  • Oncorhynchus mykiss
  • beta-Naphthoflavone
  • Environmental Chemistry
  • Environmental Science (all)
  • β-Naphthoflavone
  • 17β-Estradiol
  • Cortisol
  • Energy metabolism
  • Rainbow trout
  • 3 hydroxyacyl coenzyme A dehydrogenase
  • alpha amino acid
  • aspartate aminotransferase
  • benzo[a]pyrene
  • beta naphthoflavone
  • estradiol
  • fructose bisphosphatase
  • glucokinase
  • glucose
  • glucose 6 phosphatase
  • glucose 6 phosphate dehydrogenase
  • glutamate dehydrogenase
  • glycogen
  • hexokinase
  • hydrocortisone
  • lactate dehydrogenase
  • lactic acid
  • polycyclic aromatic hydrocarbon
  • steroid hormone
  • vegetable oil
  • amino acid
  • enzyme activity
  • hormone
  • metabolism
  • PAH
  • salmonid
  • amino acid blood level
  • amino acid metabolism
  • animal experiment
  • animal tissue
  • article
  • blood level
  • catabolism
  • controlled study
  • enzyme assay
  • estradiol blood level
  • female
  • gluconeogenesis
  • glucose blood level
  • glycogenolysis
  • hormonal regulation
  • hormone blood level
  • hydrocortisone blood level
  • immaturity
  • lactate blood level
  • liver metabolism
  • liver toxicity
  • metabolic parameters
  • nonhuman
  • sampling
  • vegetable oil fats and oils
  • Pisces Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Osteichthyes [85206] Oncorhynchus mykiss species rainbow trout common female
  • 17-beta-estradiol 50-28-2
  • 3-hydroxyacyl-coenzyme A dehydrogenase HOAD 9028-40-4 regulation
  • alpha-amino acid
  • aspartate aminotransferase 9000-97-9 regulation
  • benzo(a)pyrene BaP 50-32-8 toxin, mutagen, carcinogen
  • beta-naphthoflavone beta-NF 6051-87-2 toxin, mutagen, carcinogen
  • cortisol 50-23-7
  • fructose-1,6-bisphosphatase regulation EC 3.1.3.11
  • glucose 58367-01-4
  • glucose-6-phosphatase 9001-39-2 regulation EC 3.1.3.9
  • glucose-6-phosphate dehydrogenase 9001-40-5 regulation
  • glutamate dehydrogenase GDH 9029-12-3 regulation
  • glycogen 9005-79-2
  • lactate 113-21-3
  • 03502, Genetics - General
  • 10060, Biochemistry studies - General
  • 10067, Biochemistry studies - Sterols and steroids
  • 10068, Biochemistry studies - Carbohydrates
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 12512, Pathology - Therapy
  • 13002, Metabolism - General metabolism and metabolic pathways
  • 14004, Digestive system - Physiology and biochemistry
  • 15002, Blood - Blood and lymph studies
  • 15004, Blood - Blood cell studies
  • 22002, Pharmacology - General
  • 22501, Toxicology - General and methods
  • 24007, Neoplasms - Carcinogens and carcinogenesis
  • Ingestion and Assimilation
  • liver digestive system
  • plasma blood and lymphatics
  • Digestive System
  • Metabolism
  • Pharmacology
  • Toxicology

Fingerprint Dive into the research topics of 'beta-naphthoflavone and benzo(a)pyrene treatment affect liver intermediary metabolism and plasma cortisol levels in rainbow trout Oncorhynchus mykiss'. Together they form a unique fingerprint.

Cite this